How to check for probe specificity?

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Diana Baxter

Hi, the only way you can be 100% sure of specificity is performing the pulldown and checking what is in your pulldown.

Aissa Saidi

You should have two panels to proceed an analytical inclusivity and exclusivity tests. Your designed probe must match (inclusivity) diverse sequences containing the targeted gene, and mismatch (exclusivity) all (or the maximum) of the nearest representative relative sequences to your target gene.

example: Article Developing Inclusivity and Exclusivity Panels for Testing Di...

https://www.dnasoftware.com/wp-content/uploads/SPADA-Report-Developing-Molecular-Assays-for-Microbial-Pathogens.pdf

Yuan-Yeu Yau

Afi Gl

Not primer, then what is it?