Reviewer ask to do a flow injection analysis system or batch-injection analysis. we dont have that instruments.how to answer this question?

The authors use cyclic voltammetry and amperometry to determine H2O2. However, these electrochemical techniques are not suitable, as they were used for quantification. The amperometry in a static...

06 October 2020 7,289 2 View

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I want to use DEM(liggghts) to get soil stress-strain relationship. And then import this into abaqus soil constitutive model. How to achieve it ? Can I use python scripting or write a UMAT code?

25 December 2019 8,727 2 View

What is the mechanism for upregulation of this E3 ligase?

Some people know how ubiquitin protease ligase E3 is degraded? Perhaps this range is a bit big. If anyone knows any degradation pathway that belongs to E3 ligase, please tell me, thank you very...

19 April 2018 884 2 View

Localization a protein in cyanobacteria Synechococcus.Why can't work?

I want to localize a protein in cyanobacterial Synechococcus PCC7942.The problems I met is that there was no fluorescence whether eyfp was in N- or C- terminal of my protein. The part of my...

05 March 2018 1,467 1 View

Advice on the form of CD40L in B cell culture?

We were thinking to culture mouse B cells from the spleen in vitro for a week or two. We need them to expand and produce cytokines. I did some research on the protocols, many groups use feeder...

06 December 2017 9,801 6 View

The wrong size of protein that I want to overexpression?

I just want to overexpression a peptides in hepatoma cells is just 15 kDa, but i get the postive protein is almost 60kDa. And the peptide could be binding with a 47kDa protein. I use the thermo...

05 November 2017 1,260 3 View

How to determine the binding of two proteins to each other?

27 April 2017 9,314 5 View

Which plate is better?

I want to detect GFP fluorescence in 96-well plate. We have 2 type plate. One is black plate (corning, Cat No:3603) and the other is white plate (corning, Cat No:3610). Could you help me which one...

16 August 2016 2,517 3 View

S it necessary to check by Southern Blot except for the PCR and sequencing test?

09 June 2016 4,854 1 View

What's the effective dose of tamoxifen to induce the recombination of CreERT2 x R26EYFP in mouse embryonic liver during gestation?

06 January 2015 9,476 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Why can't make aluminum nitride film successfully by use sputter?

We use reactive sputtering process to make aluminum nitride film, and want to make c-axis aluminum nitride film on a silicon substrate, but the crystal orientation cannot be seen with...

03 March 2021 7,975 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View