Reviewer ask to do a flow injection analysis system or batch-injection analysis. we dont have that instruments.how to answer this question?

The authors use cyclic voltammetry and amperometry to determine H2O2. However, these electrochemical techniques are not suitable, as they were used for quantification. The amperometry in a static...

06 October 2020 7,289 2 View

How to change time length of tabular data in Ansys Static Structural?

Hello, Does anyone know how to change the time length to 2 or 3s in Ansys Static Structural -> Model -> Static Structural -> Pressure -> Magnitude -> Function -> Tabular data?...

10 March 2020 9,069 3 View

What is the mechanism for upregulation of this E3 ligase?

Some people know how ubiquitin protease ligase E3 is degraded? Perhaps this range is a bit big. If anyone knows any degradation pathway that belongs to E3 ligase, please tell me, thank you very...

19 April 2018 884 2 View

Localization a protein in cyanobacteria Synechococcus.Why can't work?

I want to localize a protein in cyanobacterial Synechococcus PCC7942.The problems I met is that there was no fluorescence whether eyfp was in N- or C- terminal of my protein. The part of my...

05 March 2018 1,467 1 View

Advice on the form of CD40L in B cell culture?

We were thinking to culture mouse B cells from the spleen in vitro for a week or two. We need them to expand and produce cytokines. I did some research on the protocols, many groups use feeder...

06 December 2017 9,801 6 View

The wrong size of protein that I want to overexpression?

I just want to overexpression a peptides in hepatoma cells is just 15 kDa, but i get the postive protein is almost 60kDa. And the peptide could be binding with a 47kDa protein. I use the thermo...

05 November 2017 1,260 3 View

How to determine the binding of two proteins to each other?

27 April 2017 9,314 5 View

Which plate is better?

I want to detect GFP fluorescence in 96-well plate. We have 2 type plate. One is black plate (corning, Cat No:3603) and the other is white plate (corning, Cat No:3610). Could you help me which one...

16 August 2016 2,517 3 View

S it necessary to check by Southern Blot except for the PCR and sequencing test?

09 June 2016 4,854 1 View

What's the effective dose of tamoxifen to induce the recombination of CreERT2 x R26EYFP in mouse embryonic liver during gestation?

06 January 2015 9,476 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

How to write a scientific report?

We are analysing scientific reports at University in great detail, so I wanted to find a scientific report about how to write a scientific report. Including detail such as what information to put...

02 March 2021 7,602 4 View

Which SAR dataset to use for generating coherency matrix and performing target decomposition (Description) ?

Working on chandrayaan-2 DFSAR data, there are three datasets available: 1) Slant range image data product: The slant range complex image file. Each pixel is represented by two 4-byte floating...

02 March 2021 8,481 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View