Reviewer ask to do a flow injection analysis system or batch-injection analysis. we dont have that instruments.how to answer this question?

The authors use cyclic voltammetry and amperometry to determine H2O2. However, these electrochemical techniques are not suitable, as they were used for quantification. The amperometry in a static...

06 October 2020 7,289 2 View

How to change time length of tabular data in Ansys Static Structural?

Hello, Does anyone know how to change the time length to 2 or 3s in Ansys Static Structural -> Model -> Static Structural -> Pressure -> Magnitude -> Function -> Tabular data?...

10 March 2020 9,069 3 View

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I want to use DEM(liggghts) to get soil stress-strain relationship. And then import this into abaqus soil constitutive model. How to achieve it ? Can I use python scripting or write a UMAT code?

25 December 2019 8,727 2 View

Is there any kit for oligo and antibody conjugation?

Hello~~Recently, I used expedon's kit to conjugate 5'-aminated oligo with antibody and it failed. I consulted the technician of Expedon and we can't find the reason. Is there any other kit for...

17 October 2018 7,648 5 View

What is the mechanism for upregulation of this E3 ligase?

Some people know how ubiquitin protease ligase E3 is degraded? Perhaps this range is a bit big. If anyone knows any degradation pathway that belongs to E3 ligase, please tell me, thank you very...

19 April 2018 884 2 View

Localization a protein in cyanobacteria Synechococcus.Why can't work?

I want to localize a protein in cyanobacterial Synechococcus PCC7942.The problems I met is that there was no fluorescence whether eyfp was in N- or C- terminal of my protein. The part of my...

05 March 2018 1,467 1 View

Advice on the form of CD40L in B cell culture?

We were thinking to culture mouse B cells from the spleen in vitro for a week or two. We need them to expand and produce cytokines. I did some research on the protocols, many groups use feeder...

06 December 2017 9,801 6 View

The wrong size of protein that I want to overexpression?

I just want to overexpression a peptides in hepatoma cells is just 15 kDa, but i get the postive protein is almost 60kDa. And the peptide could be binding with a 47kDa protein. I use the thermo...

05 November 2017 1,260 3 View

How to determine the binding of two proteins to each other?

27 April 2017 9,314 5 View

Which plate is better?

I want to detect GFP fluorescence in 96-well plate. We have 2 type plate. One is black plate (corning, Cat No:3603) and the other is white plate (corning, Cat No:3610). Could you help me which one...

16 August 2016 2,517 3 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

How do I determine the degree of polymerization of PEG 400 based on NMR integration?

Hello, I am working on the photocatalytic degradation of PEG 400. I would like to know if it's possible to determine the degree on polymerization based on NMR integration, or by the MW of the...

02 March 2021 6,186 3 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Can polymer concentrations be calculated based on the number average molecular weight?

Hello, I spin-cast two different polymers onto wafers and measure their height as a function of initial concentration. For polymer A, I have the weight-average molecular weight (Mw) as well as...

28 February 2021 7,882 2 View

What is the density for pva electrospining?

I'm Juan Carlos, a high school student who is working with electropinning, I made an electropinning machine with a 21g needle and a 30kv high voltage source and the distance between my needle is...

28 February 2021 298 3 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

No title

I am trying to find how to know the density needed for a pva electrospinning in a 0.6mm neddle, the electrospinning will be from top to bottom and the problem I really have is the solution because...

25 February 2021 2,615 3 View

How to reduce residual stresses in MD simulation?

I am working on a preparation of representative polymeric structures of some popular polymers, such as polyimide or PTFE for MD simulation, during which a single nitrogen or oxygen atom will be...

24 February 2021 8,105 3 View