How to change the intracellular pH in human cell line?

18 July 2019 1 1K Report

Hi All, I want to study the effect of change in intracellular pH. Can you suggest, how can I change the intracellular pH in human cell line. Is there any specific cell line suitable for such experiment?

Thanks.

Terrance Grant Johns

Chloroquine and ammonium chloride both reduce intracellular acidity.

Badges
Science topic

More Ajit Prakash's questions See All

My Cyclic voltammetry curve doesn't show cathodic peak of Cu2+ to Cu+. how to solve this problem?

I performed CV of Cu-MOF in a buffer solution of pH 8, and we did not find any peak corresponding to Cu2+ to Cu+. What is the reason behind this? Please give your valuable suggestions.

08 July 2024 2,388 4 View

Does the quantum confined effect affect the peak position of PL intensity?

The lower PL intensity for ultrathin MOF than that of bulk MOF also indicated a quantum confinement effect due to the ultrathin morphology of Cu-UMOF.

27 June 2024 4,855 0 View

The active area of the electrode is calculated based on the non-Faraday current. Why isn't the current density above or below zero?

Why isn't the current density above or below zero?

27 June 2024 9,283 2 View

How the slurry is preparing for cathodes in batteries?

13 June 2024 4,314 3 View

Geology Doctorate. Available for Post-doc and guest faculty. Please give suggestions. Anywhere in world ?

Geology Doctorate. Available for Post-doc and guest faculty. Please give suggestions. Anywhere in world.

09 June 2024 1,805 4 View

How can analyses a split plot design by SPSS?

02 June 2024 5,946 2 View

What do you mean by meadow plant?

01 June 2024 7,248 2 View

What is the actual difference between Competency-Based Education (CBE) and Outcome-Based Education (OBE)?

In any professional education students' Competency is Evaluated and used as the measurement of Outcome of the teaching-learning process. Then how these two are differentiated in curriculum...

22 May 2024 8,488 25 View

Can I purify mononucleosome from T-cell using a kit followed by SEC superdex 200 10/300 column chromatography for CryoEM ?

I am planning to purify the mononucleosome for cryoEM study. I have seen, people assemble the nucleosome from recombinantly expressed histones and then providing DNA sequence to it. Isn't this...

19 May 2024 7,170 1 View

How can I prepare a loading dye that also act as a stopping buffer for an enzymatically degraded DNA, for analysis on a sequencing gel?

I have a recipe of loading dye (10 mM EDTA, 1X TBE, pinch of Xylene cynol and Orange G dye which is made upto 10 ml with 100% Formamide) but somehow the bands of the nucleotide product...

17 April 2024 8,025 6 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to assess the osmotic power of a substance?

Hello, I suspect that a molecule I am using is causing bacteria to experience osmotic shock. What chemical properties should I look for to compare this capability with those of other substances?...

06 August 2024 977 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View