Ajit Prakash

3 Questions 1 Answers 0 Followers

Questions related from Ajit Prakash

How to change the intracellular pH in human cell line?

Hi All, I want to study the effect of change in intracellular pH. Can you suggest, how can I change the intracellular pH in human cell line. Is there any specific cell line suitable for such...

18 July 2019 1,126 1 View

Can anyone suggest a pH for my protein purification?

I want to purify my protein ( PI 7.6). When I used buffer with pH 8, my protein aggregates. What should the pH of the buffer for purifying my protein be?

12 June 2014 8,426 9 View

Which software should be used to do DNA-Protein docking?

I want to do DNA protein docking. I know important residues of the protein, involved in interaction but which part of DNA is important is not clear. Also I want to provide some flexibility for...

21 April 2014 6,633 7 View