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Hi All, I want to study the effect of change in intracellular pH. Can you suggest, how can I change the intracellular pH in human cell line. Is there any specific cell line suitable for such...
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I want to purify my protein ( PI 7.6). When I used buffer with pH 8, my protein aggregates. What should the pH of the buffer for purifying my protein be?
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I want to do DNA protein docking. I know important residues of the protein, involved in interaction but which part of DNA is important is not clear. Also I want to provide some flexibility for...
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I am doing a EMSA with a short 20 bp oligo. I got complementary strands synthesized and annealed them. After annealing I run a gel and purified oligo from gel. But the yield after purification is...
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