We are trying to calculate the lipophilicity of a peripheral protein?
Thank you very much for your information. I want to calculate the lipophilicity of the protein. Could you please suggest me the correct software/server for this purpose?
I deposited TiO2 thin film on Si substrate from a TiO2 oxide target using rf magnetron sputtering technique (only Ar plasma is used ). Can i expect individual Ti and O2 atoms are sputtered from...
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I deposited a very thin(~2nm) TiO2 film on Si substrate by magnetron sputtering. After that i did XPS of this film at room temperature and got following spectra. Can anyone know why an extra peak...
19 May 2024 3,018 4 View
Why we called it re-combination but not combination?
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Cosine similarity, Soft Cosine similarity or SBERT?
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"Awaiting Referee Scores" means ?
23 June 2023 8,946 6 View
I was using the following input lines: %nprocshared=16 %mem=47GB %oldchk=Restarting_with_1TS2.chk %chk=gv5_Restarting_with_1TS2.chk #p stable=opt lanl2dz guess=read geom=check m06 But I get an...
15 June 2023 1,314 1 View
I have been trying to get an optimized structure of transition state confirmed with IRC but if says exceeding maximum corrector steps so is there any other way that I can rtack the starting...
04 June 2023 3,300 3 View
Good Morning all. I am trying to use U_mass coherence score to select the best number of topics in LDA model. To my understanding, for u_mass bigger score is better. But in this case -3 or -7,...
01 June 2023 5,578 0 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
why don't d-orbitals split themselves because of themselves without the presence of ligands? Electrons are indistinguishable. Why wouldn't it be more correct that protons from a ligand split the...
03 August 2024 3,589 3 View
I'm guessing it's because the ligand experiences too much electron repulsion or proton repulsion from the chromium to insert them close to the 3d-orbitals which are close to the metal nucleus. Is...
03 August 2024 1,370 1 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello,, The metal complex ligand appears incomplete in the screen of the discovery studio visualizer
30 July 2024 3,354 1 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View