Why the storage moduli E' decrease with highr fiber volume?

Hi guys, I am facing problems to interpret a DMA results. Material: Composite Bisphenol A + curaua fiber What is being tested: the influence of fiber volume on the composites properties...

12 February 2021 174 7 View

Which buffer should I use to aliquot antibody in?

for western blot analysis the buffer in which the antibody already is, written in the datasheet, or should I use already the bsa or milk solution that I use for final diluition?

17 November 2020 4,804 3 View

What is the best protocol for simultaneous extraction and purification of DNA and RNA from animal tissues?

I need to perform a co-extraction of DNA and RNA from pools of juvenile Daphnia magna. I used the AllPrep DNA/RNA Mini Kit (QIAGEN) but with poor results in terms of DNA yeld and quality. I would...

22 October 2020 9,746 3 View

No title

The threat of pandemic disease is not new: For decades, public health experts advised that a coronavirus outbreak was looming, global proportions, and that the world was not equipped to fight it...

27 May 2020 2,871 25 View

Is it correct to compare Fst values obtained from different genetic markers?

Fst is used in many genetic studies which involve different markers (mtDNA, microsatellites, SNPs...) as methodologies and next-generation genetics improve. I was wondering if it would be correct...

27 April 2020 7,175 3 View

My work entails comparing the effect of tax on economic growth of two countries. Asides the panel regression, which other technique can I use?

MY specific objective is to make comparative analysis on the effect of tax revenue on GDP of country A and country B

26 June 2019 6,619 3 View

In Morris water maze training results for scopolamine-injected rats, is it normal that scopolamine doesn't cause difference on day 5 ?

And if so, is it related to short term memory or inability of scopolamine to impair spatial learning?

17 June 2019 9,461 3 View

How to prepare aqueous solution of Methyl Jasmonate dissolved in Tween 20?

I need to prepare 500 mL of aqueous solution of 1mM Methyl Jasmonate dissolved in 0.1% of Tween 20 for foliar application. How much MJ and how much Tween 20 do I need to use? Both compounds are...

02 May 2019 6,228 2 View

May I express a protein in C. glutamicum using a commercialy availabe vector?

29 March 2019 7,700 1 View

How can i use ethidium bromide for sample staining?

I don't want to add it directly into the gel but just using it as loading dye.

25 March 2019 5,889 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View