I have used Image J software to get fluorescent intensity and got the values for Mean as 13,16,18, etc and Integral density as 3500000, 4600000, etc at different concentrations. Do I need to plot the integral density or the mean on y-axis? Also, does increase in mean or integral density signifies increase in ROS production?
Kindly me suggest me how to plot graph based on the values.
Thank you