Can you simply get the raw decay ASCII and the raw IRF ASCII?

If so, you can simply use Matlab (or other) to fit a convolution of a theoretical bi-exponential function with your experimental IRF to your experimental decay?

i(t) = sum{a(j)*exp[-t/tau(j)]}

where a(j) and tau(j) are your amplitude and lifetime components,

Then minimize the following function:

{conv [i(t), IRF(t)] - I(t)}

where IRF(t) and I(t) are your experimental IRF and decays.

One free matlab code that allows you to perform the fit is called DecayFit (http://www.fluortools.com/software/decayfit), however it is very easy to implement.

Thank you very much Eitan. Let me try based on your text and link.

you can draw your datas in ORIGINpro software. then fitting with bi exponential function. the result will give out the AMPLITUDE AND LIFETIME. GOOD LUCK.

YOU click on your plotted curve, then click ANALYSIS-> FITTING-> EXPONIENTIAL FITTING then you can chose single, bi, or three exponential funtion for fitting.

You can contact me via email [email protected]

I have fitted the TRPL for finding the life time using originpro  

Thank you khoi.

Regarding the last recommendation, this is good only if you perform a "tail fit" (fitting the decay after the rise time. This procedure is approximate and is good in cases where both your two lifetime components are longer relative to your IRF. The best approach is to reconvolute your IRF with the theoretical bi-exp function.

Peter, thanks for that.

This is another aspect I forgot to mention.

Jayaramakrishnan Velusamy, in your implementation, don't forget to weigh your fit nonuniformly, by poisson weights.

If I recall, the script I recommended includes this and much more.

similar question, i'm doing the bi-exponential fitting of my lifetime data by origin 9. For the fitting results, amplitudes, A1=0.19 and A2=2.23.

My question is why A1 and A2 not in percentage?,

i found that in literature the amplitudes were always shown in percentage.

Thanks a lot!