How to calculate synergism in western blot for demonstrating the downregulation of a protein in combo treatment is synergistic than individual drugs?

24 January 2020 0 2K Report

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How to do image processing?

I want to analyze some images (nearly 1000) in a loop. I want to analyze HSV and RGB. I have masked those images in ImageJ, which is binary masked. I tried to explore them in R, but all the...

04 June 2024 5,098 1 View

In Borylation reaction, we use Pd(dppf)2Cl2 as Pd2+ although in the reaction cycle, Pd(0) is the active species. Which reagent reduces Pd2+ ?

Which reagent reduces Pd(2+) to Pd(0) in Borylation reaction? What is the advantage of using Pd(2+) instead of Pd(0)?

27 June 2022 9,184 0 View

How to include van der waals effect in PBEsol functional ?

I have a layered structure and I want to include van der Waals effect in optimization calculation. I ran calculation with GGA=PS and then added tag VDW_S6=0.75 and ran calculation again. I see no...

28 May 2022 7,811 1 View

I want to learn FLAC software so as to implement it in numerical fracture modelling..Anyone please help me out ?

As a beginner don’t able to understand the FISH language in FLAC …how to incorporate the example can anyone guide me ?

05 November 2021 1,548 6 View

How to calculate Spontaneous polarsation using Berry phase in Quantum espresso ?

I want to calculate Sp. Polarisation and direction for the rhombohedral structure of BaTiO3. Can anyone help me with any sample input file for the calculation of polarisation? And please explain...

19 September 2021 6,509 1 View

Why covalent nature of bonds leads to FE instability , at least how much hybridization is needed to cause instability ?

In a paper, it was mentioned that the density of states shows considerable hybridization among Ti 3d, Bi 6p, and O 2p states indicating covalent nature of the bonds leading to ferroelectric...

20 July 2021 8,751 1 View

Does DOLS deal with cross sectional dependence?

Hello researchcers Can you help me to understand that the DOLS model is suitable for cross sectional dependence panel data or not. The sample of my research is CSD through individuals...

28 August 2020 3,276 5 View

Project Position at IIT Kharagpur?

ADVERTISEMENT NO. : IIT/SRIC/R/USI_UDT/2019/103, DATED June 13, 2019 Applications are invited on plain paper for the following assignment in a purely time bound research project undertaken in the...

23 June 2019 2,972 0 View

So, I am currently working on a compound called Na[CoFe(CN)6](Cobalt Hexacyanoferrate). How do I draw this molecule In Gaussian09 for GaussJobs?

In the structure of Na[CoHCF[ every CN is ligated to Fe and Co and vice versa creating kind of a continuous polymer like structure. I have attached picture of a monomer(if you will call it so).

25 May 2017 1,469 4 View

What is the main difference between the Frechet derivative and the ordinary derivative?

In the peridynamic state based model, the Frechet derivative is used, I would like to know why is this derivative is used, instead of the Gateaux or the ordinary derivative?

09 July 2015 8,699 11 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

Is changing the medium during the experiment overestimate the results?

I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...

07 August 2024 2,283 2 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,725 2 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View

How to maintain the drug concentration in cell culture study for long term?

I am working in the filed of long acting drugs with Nano formulation. I want to study the efficacy of my formulation in 2D cell cultures while this required media change on every two days. In such...

24 July 2024 8,696 0 View

How can I determine if a drug binds to a ribosome using a Cryo-EM map?

Hello, I am searching for the binding position of a drug in a ribosome, based on previous work indicating it binds there. The resolution of the ribosome-drug Cryo-EM map is around 2.5 Å. I've been...

19 July 2024 2,155 0 View

How can the economic value of digestate management, utilization, and regulation, can spark interesting discussions and explorations?

How can the economic value of digestate management, utilization, and regulation, can spark interesting discussions and explorations? so one can list down the details and we can discuss in length...

17 July 2024 3,255 4 View

How to calculate percentage cumulative drug release?

How to calculate percentage cumulative drug release when, Total volume of dissolution media = 250ml Added formulation was equivalent to 10mg of drug Sample withdrawn = 1ml each time Sink condition...

15 July 2024 4,192 1 View

MTT assay - 6 well plate?

Hi, In the 6 well plate, when I am seeding the cells. Do I add 1ml of complete media to one of the wells for the control at this stage or do I wait unit I am treating the plates to do that? Also...

13 July 2024 9,783 1 View

How to convince decision makers to adjust the legislation to allow opening of drug consumption rooms?

In my country, there is a lot of public outcry in relation to the presence of drug injection and related behaviors in public. Hence at least the bigger cities would probably benefit from the...

08 July 2024 8,903 1 View