Hi
I would like to know if anyone knows how to calculate pancreatic lipase inhibition. From the papers I saw, the calculation was done based on this formula
ABc-ABs divided by ABc and multiply by 100, where ABc is the absorbance of the control and ABs is the absorbance of the samples. This method did not work for me because it gave me a negative result.
For my own calculation, I first find the log of the absorbance of both control and samples before using the formula I mentioned above. I just want to be sure if what I did was right or wrong. Please I need more explanation on this.
I would also like to know if anyone can give me the method for the kinetics of enzyme inhibition for pancreatic lipase. I did it but all my values are negatives even at lower substrate concentration. I don't know if I am doing something wrong.
Thank you.
Hi there,
I guess you are using the colorimetric assay which quantify the release of glycerol. If ABc represents the product formed in absence of inhibitor and ABs the product formed in the presence of inhibitor then ABs should be less than ABc, otherwise the inhibitor is not actually an inhibitor. And ((ABc-ABs)/ABc) is the percentage of inhibition.
Thank you Dominique for the answer. This formula did not work for me that is why I used the log of my absorbance which I can't say is valid.
All my results are negative without the log.
I will try to look into my enzyme, maybe it is no longer active.
Hi again, you can't expect a decrease in OD as it is suppose to be relevant of the production of glycerol. You should have a careful check at your assay and reagents.
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