How to calculate nm of enzyme in a ABTS/HRP antioxidant activity assay, where the HRP has UNITS 150U/mg?

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Farjam Goudarzi

Usually the enzyme value is expressed as IU (U). However, some paper stated it as nM. HRP is 44 kDa protein that is equivalent to M.W., so you can use this below link to calculate it in accordance to volume needed.

https://www.graphpad.com/quickcalcs/Molarityform.cfm

Sonia Kumar

You resolved the problem. Thank you.