Sonia Kumar

3 Questions 2 Answers 0 Followers

Questions related from Sonia Kumar

What method can be utilized to determine the amount, ratio, and percentage of trypsin Enzyme required for complete hydrolysis of protein substrate?

I am new to the enzyme and protein field of research. Therefore I am struggling with the following issues- How to calculate the trypsin enzyme amount required for performing the complete...

06 June 2019 5,724 1 View

How to calculate nm of enzyme in a ABTS/HRP antioxidant activity assay, where the HRP has UNITS 150U/mg?

The article I am trying to follow says - The ABTS oxidation medium contained 1 mM ABTS, 45 mM hydrogen peroxide and 3 nM HRP in 50 mM acetate buffer (pH 5). I want to use Citrate phosphate...

07 July 2018 5,899 2 View

Can the molarity of a solute, be affected by the molarity of the solvent used in series dilution?

I have a Salt (548.7 g/mol) that is required to be dissolved in a buffer solution of 0.16 M citrate phosphate, pH = 5.0. I was thinking the salt has its own molarity, and the solvent in which I...

07 July 2018 5,318 1 View