Dear Masood,

You would want to make your 1.37x10^10 pfu/mL phage lysate dilution as the same as your bacterium culture, to 1.2x10^7.

so both are at 1.2x10^7 and mix 1:1

My math may be wrong.... I did on phone fast but concept is more important.....

Lets say you want to make 1 mL of phage lysate to use for your infection:

a. (1.2x10^7) / (1.37x10^10) = 0.0008759mL

b. 0.0008759mL*1000 = 0.8759uL .... too small to pipet

c. thus do a 1:100 dilution of your lysate...

d. then ad 87.59uL of 1:100 diluted lysate to 912.41uL of medium.

e. add at volume of one to one with bacterium, mix well for moi of 1:1

Charles D Anderson Thankyou so much for detailed answer

You can calculate using a simple system like this:

1.37x10^10 x (MOI) x a =1.2x10^7 X b

a + b = "Final Volume"

Solving the system, "a" and "b" will be the volumes you must add to get the desired MOI in the final volume that you choice.