Saleha Masood

13 Questions 15 Answers 0 Followers

Questions related from Saleha Masood

How to confirm that you have assembled the contigs of your sequenced genome correctly?

I have sequenced my phage genome from Qiagen. I'm using Galaxy CPT for genome assembly and annotation. My Question is that How can I confirm that I have assembled the contigs of my phage...

28 April 2022 9,288 3 View

Size marker for TEM images of Phage?

Hey, I have done the Transmission Electron Microscopy of my phages and got nice images. Now i've classified my phages depending on their morphology. In many papers, i have seen they have measured...

14 March 2021 5,805 1 View

How to obtain plaques of phages by double layer agar assay?

I'm performing double layer agar assay for my phages. The protocol I'm following is 1- culture the bacteria in LB broth until the exponential phase is attained 2- Make dilutions of the phage i-e,...

08 January 2021 4,628 6 View

Bacterial Growth Reduction by Phage?

Hi, I have been doing bacterial growth reduction assay of my phages recently. In all the papers, i have seen that O.D of cultures with added phages (at different MOIs) increase at first ( for...

25 October 2020 9,796 2 View

What is the difference between multiplicity of infection and optimal multiplicity of infection?

I have to perform an experiment "lysis profile of host bacteria after phage infection" in which i will check the reduction in bacterial growth at different MOIs (0.1, 1. 10) by taking O.D600 ....

21 September 2020 3,229 1 View

How the ratio of phage resistant strain(%) is calculated?

Hi, I'm working on phage therapy. In bacterial growth reduction assay, the O.D decreases during first 8 hours and then start to increase which maybe due to cell debris or emerging phage resistant...

12 March 2020 6,711 5 View

Rapid identification of phages through multiplex PCR?

Hi, I’m currently working on phages against pathogenic E.coli and I have come across this article of rapid identification of selected phages through Multiplex PCR. This method allows detection at...

08 March 2020 2,077 4 View

How to calculate multiplicity of infection(MOI)?

Hi, I have to perform bacterial growth reduction assay and I have a confusion in calculating MOI(multiplicity of infection). PFU of phage is 1.37×1010pfu/ml. How much of phage lysate do I have to...

05 March 2020 4,657 3 View

Multiplicity of infection

Hi.. i have to perform " lysis profile of host bacteria after phage infection". In a paper, they have taken 0.1 MOI in this assay. I want to ask on what basis they have selected only one MOI...

01 January 1970 4,631 3 View

Calculating the volume of bacteria

Hi, I have calculated the cfu/ml of bacteria by serial dilution method ( by spreading 100ul of diluted bacterial culture on N-agar plates and counting the no. of colonies after overnight...

01 January 1970 6,903 5 View

Extraction of phage DNA for whole genome sequencing

Hi, I want to send my samples of phage for whole genome sequencing as we don't have the facility of WGS in our institute. I have extracted the DNA of phages by ZnCl2 precipitation method...

01 January 1970 5,429 2 View

Biofilm removal capacity of phages

Hey, I want to check my phages against the bacterial biofilm. I have read papers in which they have added phages in a specific MOI (0.6, 1 etc) either in pretreatment or post treatment of...

01 January 1970 4,399 7 View

Query regarding Whole Genome Sequencing

Hi, Can anyone suggest a good company from where i can sequence my phage genomes. I want to go for Microbes NG but have heard some bad reviews about them. Considering i'm from pakistan, please...

01 January 1970 2,776 3 View