I am doing whole cell recordings of AMPA or Kainate receptors expressed in HEK cells. My best fittings of desensitization curve with "exponential standard" (Clampfit), two term fitting. How to calculate the mean weighted values of Tau 1 and Tau2.
If possible please give an example for the same.
Best,
Rajesh Vinnakota
Hi Rajesh,
The tau given in Clampfit Results window is already an estimation of the mean tau value for the given trace. If you would like a measure of its reproducibility for the same cell, repeat the current excitation for 3/5 times, spaced by at least two minutes of recovery. Tau values are pretty stable if the channels aren't forced to desensitize. For nAChRs it works very well, with good iter-cell reproducibilty.
Best,
Giulia
a exp(-t/t1) + b exp(-t/t2)
t1 and t2 are the time constants and a and b are the "weights".
The weighted decay time constant is:
tw = (a*t1+b*t2)/(a+b)
Thanks Victor,
But how to give "weights" for tau values.
for example Tau1 is 3.7 ms and Tau2 is 1.2 ms.
Rajesh, That comes directly from the fit, it gives you the weight and the tau.
Dong,
When a function does not show simple exponential decay, but rather has several terms each with a different time constant, then each term has a "weight" associated with it.
The "weighted" time constant is a way to describe the process with a single value for comparison
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