Rajesh Vinnakota

11 Questions 21 Answers 0 Followers

Questions related from Rajesh Vinnakota

Is there any specific amplifier settings recommended for Single Channel and Pulled Patch experiments?

Hi, I am using HEKA USB 10 amplifier for my whole-cell patch-clamp experiments. I would like to perform Single-channel recordings and outside-out (Pulled) recordings with the same Patch rig. Now I...

18 February 2021 6,172 2 View

How to plot a rectification index curve (I/V plot)?

HEK cell is Clamped at different membrane potentials (-100 mV to +90 mV; 10 mv Step with Vh -60 mV) of GluK2 receptor and recorded the current by application of 10 mM L-Glutamate. Herewith I...

08 December 2020 2,282 1 View

I would like to know the Xenopus Oocyte approximate membrane thickness?

Hi, I would like to know the Xenopus Oocyte approximate membrane thickness? Suggest me please. Best, Rajesh

02 August 2019 5,920 1 View

How to calculate " Mean Weighted Tau Desensitization" in two term fitting?

I am doing whole cell recordings of AMPA or Kainate receptors expressed in HEK cells. My best fittings of desensitization curve with "exponential standard" (Clampfit), two term fitting. How to...

25 January 2019 1,186 7 View

Hi, I am struggling With NMDA receptor Electrophysiology studies....?

Hi, I am just curious to know about the NMDA receptor patch clamp studies. There are huge number of research papers are available and plethora of information available on NMDA receptor. Very...

19 January 2018 1,546 8 View

I stuck with fitting the Desensitization (Tau) kinetics for ionotropic glutamate receptor currents...?

Hi, I am working on Ionotropic glutamate receptors. Here I attached 100 ms, desensitized currents of GluR 6 Native with 10 mM Glutamate (abf) file. Please help to fit for desensitization...

15 January 2018 9,350 4 View

Is it possible to block N-Type inactivation with TEA in bath solution (whole cell)?

If channel is potassium selective, Is it possible to block N-type inactivation with TEA in bath solution on whole cell mode conventional patch clamp. If yes can anyone suggest any reference for...

08 December 2015 8,583 3 View

Can anyone help me with rice stomatal density?

I counted the stomatal densities of rice adaxial leaf (vegetative stage), in mm2 (stomatal imprint method) I was found that it has less than 100 stomata, but rice generally having high amount of...

17 November 2015 3,358 4 View

Can anyone suggest Lab bench and other settings for current clamp (Action potential) pClamp software?

Hi, Can anyone suggest that Lab bench and other settings for current clamp (Action potential) pClamp software. If I am setting the Lab bench Input settings mV for IN=0, the popup showing that "bad...

26 August 2015 5,813 3 View

Does anyone know a good method for determination of plant transpiration?

I want to know the transpiration rate between various rice varieties.

02 February 2014 4,981 4 View

How to calculate "p" value from 95 % confidence interval of two sets of data?

How to calculate p value from 95 % confidence interval of two sets of data? Please suggest the way to calculate the same with Origin or Prism. Data 1 ________ Mean 0.787 95 %CI 0.7086v to...

01 January 1970 4,570 5 View