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Questions related from Rajesh Vinnakota
Hi, I am using HEKA USB 10 amplifier for my whole-cell patch-clamp experiments. I would like to perform Single-channel recordings and outside-out (Pulled) recordings with the same Patch rig. Now I...
18 February 2021 6,126 2 View
HEK cell is Clamped at different membrane potentials (-100 mV to +90 mV; 10 mv Step with Vh -60 mV) of GluK2 receptor and recorded the current by application of 10 mM L-Glutamate. Herewith I...
08 December 2020 2,178 1 View
I am doing whole cell recordings of AMPA or Kainate receptors expressed in HEK cells. My best fittings of desensitization curve with "exponential standard" (Clampfit), two term fitting. How to...
25 January 2019 1,122 7 View
Hi, I am just curious to know about the NMDA receptor patch clamp studies. There are huge number of research papers are available and plethora of information available on NMDA receptor. Very...
19 January 2018 1,503 8 View
Hi, I am working on Ionotropic glutamate receptors. Here I attached 100 ms, desensitized currents of GluR 6 Native with 10 mM Glutamate (abf) file. Please help to fit for desensitization...
15 January 2018 9,301 4 View
Hi, Can anyone suggest that Lab bench and other settings for current clamp (Action potential) pClamp software. If I am setting the Lab bench Input settings mV for IN=0, the popup showing that "bad...
26 August 2015 5,764 3 View
How to calculate p value from 95 % confidence interval of two sets of data? Please suggest the way to calculate the same with Origin or Prism. Data 1 ________ Mean 0.787 95 %CI 0.7086v to...
01 January 1970 4,500 5 View