How much is the time gap between a intrinsic action potential and a postsynaptic potential?

Hi everyone for example, there are two neurons. One is presynaptic neuron (neuron A) and one is postsynaptic neuron (neuron B), which means they are mono synaptically connected and no feedback...

11 December 2018 3,227 0 View

Can I say both LTP and LTD depend on influx Ca2+, but the amount of delta[Ca2+]i leads to different results?

It is very helpful to me if anything I wrote is wrong is pointed out. In this way I can learn more or correct what I knew. Many thanks. As far as I understand, LTD is Ca2+ influx but activates...

04 May 2018 7,895 1 View

How to load neuron cells using fluo4am?

we can mainly loading glial cell sigh fluo4am. we wonder if there is any tip to make fluo4am loaded mainly into neuron cells. or if there is any other proper Ca2+ dye for neurons. the age of the...

03 April 2018 1,226 3 View

Is Cal 520 AM labling neuron or glial cell?

any one ever worked with this dye before? any suggestion is helpful. thx

03 April 2018 202 2 View

What is the Confidence graph's min/max?

Hi, after I search a gene in pubmed, in the genome region graphic there are two green colors (dark and light). As website defines, there are "Confidence graph's minimum" and "Confidence graph's...

02 March 2018 3,325 0 View

What is the difference between headstage and bipolar electrode on making field stimulation?

for example, I am going to give a field stimulation (square-wave pulses of 100 mV 1000Hz duration 1ms). and I have a axon patch 700B with two headstage and one pencilshapged concentric bipolar...

01 February 2018 3,868 1 View

Can Mowiol medium work with fluoro-gold?

10 November 2016 6,648 0 View

Why both de- or hyper-polarisation DECREASE action potential-induced calcium-dependent fluorescence transient?

In this review (PMID: 15471589, section 2.4), it saids the "action potential-induced calcium-dependent fluorescence transient" at axonal boutons is related to membrane potential. NO matter de- or...

01 January 1970 2,822 3 View

Sutter electrode holder leaking after applying positive pressure. How to fix?

I am using a Sutter electrode holder for my patch clamp experiments, but I'm having trouble keeping positive pressure inside my electrode due to a leak out of the back of the holder. I have added...

02 March 2021 5,829 1 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

What thickness need for ITO to use as conductive layer?

I want to do RF sputtering of ITO on glass substrate using magnetron sputtering. what thickness do i need to make it good conductive layer to use it for PSC. Moreover, whats about thickness of...

01 March 2021 395 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Which group formation theory do you recommend for projects that aim to bring together entrepreneurs for training for regional development?

I would like to have ideas about which theories of group formation are suitable for entrepreneurial training projects, with the aim of creating bonds, building trust, and collaboration for...

28 February 2021 4,223 2 View

Why is my platinum counter electrode getting dirty during electrodeposition?

I am conducting anodic electrodeposition of MnO2 onto a stainless steel substrate, using a platinum strip as a counter electrode. This is done galvanostatically, with the current held between 5...

27 February 2021 1,829 3 View

Can I run spearman's correlation on non monotonic data? Is it advisable?

Hi all, I am running a partial correlation analysis on mood data. I want to see if there is a correlation between scores on a novel hypomanic mood scale and existing measures of hypomania - the...

25 February 2021 1,619 3 View

How does charge on working electrode changes with rising potential?

During performing CV of a GCE or modified GCE, we rise the potential in the first half of the cycle and reduce it in reverse half. During the first half, what is the nature of charge on GCE or...

25 February 2021 7,994 3 View

How to handle studies which report perfect effect sizes in a meta-analysis using Pearson's r?

I'm in the process of updating a meta-analysis and in one of the new studies, effect sizes are reported as Tau-U. Two of the effect sizes are reported as Tau-U = 1.00. I am using Andy Field's...

24 February 2021 2,265 3 View