How much is the time gap between a intrinsic action potential and a postsynaptic potential?

Hi everyone for example, there are two neurons. One is presynaptic neuron (neuron A) and one is postsynaptic neuron (neuron B), which means they are mono synaptically connected and no feedback...

11 December 2018 3,227 0 View

Can I say both LTP and LTD depend on influx Ca2+, but the amount of delta[Ca2+]i leads to different results?

It is very helpful to me if anything I wrote is wrong is pointed out. In this way I can learn more or correct what I knew. Many thanks. As far as I understand, LTD is Ca2+ influx but activates...

04 May 2018 7,895 1 View

How to load neuron cells using fluo4am?

we can mainly loading glial cell sigh fluo4am. we wonder if there is any tip to make fluo4am loaded mainly into neuron cells. or if there is any other proper Ca2+ dye for neurons. the age of the...

03 April 2018 1,226 3 View

Is Cal 520 AM labling neuron or glial cell?

any one ever worked with this dye before? any suggestion is helpful. thx

03 April 2018 202 2 View

How to calculate L-ratio? define the threshold for the spike training result?

Hi, we used multiple electrode to detect neuronal activity and we got several spike trains. what we dont know is how to 1, define the proper threshold to get rid of most of the noise? 2,...

02 March 2018 8,711 1 View

What is the difference between headstage and bipolar electrode on making field stimulation?

for example, I am going to give a field stimulation (square-wave pulses of 100 mV 1000Hz duration 1ms). and I have a axon patch 700B with two headstage and one pencilshapged concentric bipolar...

01 February 2018 3,868 1 View

Can Mowiol medium work with fluoro-gold?

10 November 2016 6,648 0 View

Why both de- or hyper-polarisation DECREASE action potential-induced calcium-dependent fluorescence transient?

In this review (PMID: 15471589, section 2.4), it saids the "action potential-induced calcium-dependent fluorescence transient" at axonal boutons is related to membrane potential. NO matter de- or...

01 January 1970 2,822 3 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

How can I make stacked bar graph with scatter plot?

I want to make a stacked bar graph with showing each data value as dots. I tried with graph pad prism, version 6, but could not succeed. Does anyone have tried before making such graphs?

01 March 2021 9,383 1 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

What could be reason for getting such type of graph for complex impedance in case of polymer blend films?

Its effect appears in real part of ac conductivity also. Can anyone suggest any reason behind it?

26 February 2021 3,487 3 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

Can I use a graph from a another source in a thesis?

I have a permission from X company to use their graph from a report, but they have a reference in the bottom left corner of the graph as follows: "Source: Database Y". Is it OK to use this graph...

24 February 2021 2,269 3 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

I have need of XRD graph analysis software for phase identification. Can you please provide links for software tools which can be downloaded?

i am trying to download x'pert high score software, but can't download it. please do suggest software for phase analysis

24 February 2021 5,687 4 View