At the website of fluorochrome, it suggest to use neutral buffered glycerine. Does it mean any PH=7 glycerol mounting media is OK to work with FLUORO-GOLD? I normally use homemade Mowiol. Anyone ever tried whether this medium is OK to use? THANKS!
Hi everyone for example, there are two neurons. One is presynaptic neuron (neuron A) and one is postsynaptic neuron (neuron B), which means they are mono synaptically connected and no feedback...
11 December 2018 3,227 0 View
It is very helpful to me if anything I wrote is wrong is pointed out. In this way I can learn more or correct what I knew. Many thanks. As far as I understand, LTD is Ca2+ influx but activates...
04 May 2018 7,895 1 View
we can mainly loading glial cell sigh fluo4am. we wonder if there is any tip to make fluo4am loaded mainly into neuron cells. or if there is any other proper Ca2+ dye for neurons. the age of the...
03 April 2018 1,226 3 View
any one ever worked with this dye before? any suggestion is helpful. thx
03 April 2018 202 2 View
Hi, we used multiple electrode to detect neuronal activity and we got several spike trains. what we dont know is how to 1, define the proper threshold to get rid of most of the noise? 2,...
02 March 2018 8,711 1 View
Hi, after I search a gene in pubmed, in the genome region graphic there are two green colors (dark and light). As website defines, there are "Confidence graph's minimum" and "Confidence graph's...
02 March 2018 3,325 0 View
for example, I am going to give a field stimulation (square-wave pulses of 100 mV 1000Hz duration 1ms). and I have a axon patch 700B with two headstage and one pencilshapged concentric bipolar...
01 February 2018 3,868 1 View
In this review (PMID: 15471589, section 2.4), it saids the "action potential-induced calcium-dependent fluorescence transient" at axonal boutons is related to membrane potential. NO matter de- or...
01 January 1970 2,822 3 View
Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...
03 March 2021 1,499 2 View
Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...
03 March 2021 6,299 3 View
I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.
02 March 2021 7,735 3 View
For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...
01 March 2021 5,035 2 View
Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...
01 March 2021 2,215 2 View
Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.
01 March 2021 9,952 3 View
I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...
01 March 2021 9,525 4 View
I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...
01 March 2021 9,015 2 View
I have found only four of them; www.surveyplanet.com www.online-anket.gen.tr www.surveey.com and of course google forms. Expecting to see your suggestions if there is any. Thank you
28 February 2021 3,237 3 View
I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...
26 February 2021 7,129 3 View