At the website of fluorochrome, it suggest to use neutral buffered glycerine. Does it mean any PH=7 glycerol mounting media is OK to work with FLUORO-GOLD? I normally use homemade Mowiol. Anyone ever tried whether this medium is OK to use? THANKS!
Hi everyone for example, there are two neurons. One is presynaptic neuron (neuron A) and one is postsynaptic neuron (neuron B), which means they are mono synaptically connected and no feedback...
11 December 2018 3,248 0 View
It is very helpful to me if anything I wrote is wrong is pointed out. In this way I can learn more or correct what I knew. Many thanks. As far as I understand, LTD is Ca2+ influx but activates...
04 May 2018 7,920 1 View
we can mainly loading glial cell sigh fluo4am. we wonder if there is any tip to make fluo4am loaded mainly into neuron cells. or if there is any other proper Ca2+ dye for neurons. the age of the...
03 April 2018 1,265 3 View
any one ever worked with this dye before? any suggestion is helpful. thx
03 April 2018 242 2 View
Hi, after I search a gene in pubmed, in the genome region graphic there are two green colors (dark and light). As website defines, there are "Confidence graph's minimum" and "Confidence graph's...
02 March 2018 3,353 0 View
Hi, we used multiple electrode to detect neuronal activity and we got several spike trains. what we dont know is how to 1, define the proper threshold to get rid of most of the noise? 2,...
02 March 2018 8,739 1 View
for example, I am going to give a field stimulation (square-wave pulses of 100 mV 1000Hz duration 1ms). and I have a axon patch 700B with two headstage and one pencilshapged concentric bipolar...
01 February 2018 3,933 1 View
In this review (PMID: 15471589, section 2.4), it saids the "action potential-induced calcium-dependent fluorescence transient" at axonal boutons is related to membrane potential. NO matter de- or...
01 January 1970 2,852 3 View
Is this website real? https://isar.org.in/event/registration.php?id=2434532
08 August 2024 484 1 View
A website software of Blackbody radiation law expert software can used through the following web site. http://39.105.188.151:3000/index
07 August 2024 1,706 0 View
I have face this problem anyone help me how to solve this issue ?which is below Fatal error: There are inconsistent shifts over periodic boundaries in a molecule type consisting of 78 atoms. The...
07 August 2024 2,598 1 View
Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?
07 August 2024 4,616 0 View
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,373 2 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
Dear friends, does anybody know that is it legal to upload a graphical abstract previously published on your paper's first page to a website such as figshare.com under CC-BY-4.0 license? Thanks.
05 August 2024 7,098 3 View
Reference dose and Maximum acceptable concentrations HMs
03 August 2024 8,230 4 View
Molecular docking software/ websites?
02 August 2024 8,704 7 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View