Can I say both LTP and LTD depend on influx Ca2+, but the amount of delta[Ca2+]i leads to different results?

It is very helpful to me if anything I wrote is wrong is pointed out. In this way I can learn more or correct what I knew. Many thanks. As far as I understand, LTD is Ca2+ influx but activates...

04 May 2018 7,895 1 View

How to load neuron cells using fluo4am?

we can mainly loading glial cell sigh fluo4am. we wonder if there is any tip to make fluo4am loaded mainly into neuron cells. or if there is any other proper Ca2+ dye for neurons. the age of the...

03 April 2018 1,226 3 View

Is Cal 520 AM labling neuron or glial cell?

any one ever worked with this dye before? any suggestion is helpful. thx

03 April 2018 202 2 View

How to calculate L-ratio? define the threshold for the spike training result?

Hi, we used multiple electrode to detect neuronal activity and we got several spike trains. what we dont know is how to 1, define the proper threshold to get rid of most of the noise? 2,...

02 March 2018 8,711 1 View

What is the Confidence graph's min/max?

Hi, after I search a gene in pubmed, in the genome region graphic there are two green colors (dark and light). As website defines, there are "Confidence graph's minimum" and "Confidence graph's...

02 March 2018 3,325 0 View

What is the difference between headstage and bipolar electrode on making field stimulation?

for example, I am going to give a field stimulation (square-wave pulses of 100 mV 1000Hz duration 1ms). and I have a axon patch 700B with two headstage and one pencilshapged concentric bipolar...

01 February 2018 3,868 1 View

Can Mowiol medium work with fluoro-gold?

10 November 2016 6,648 0 View

Why both de- or hyper-polarisation DECREASE action potential-induced calcium-dependent fluorescence transient?

In this review (PMID: 15471589, section 2.4), it saids the "action potential-induced calcium-dependent fluorescence transient" at axonal boutons is related to membrane potential. NO matter de- or...

01 January 1970 2,822 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

No title

Hi, I have several doubts about one paper (Systematic Literature Review) that was rejected. The main topic is Single-Board Computers in engineering education during the period 2010-2020. The...

01 March 2021 9,015 3 View

How to model the battery pack in PSIM or PSCAD?

Hello, I have simulated a single battery cell using Thevenin equivalent circuit. For a battery pack, I have a large number of cells connected in series and parallel connections. Is there any way...

28 February 2021 2,116 1 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

How to find the right number of integration points in second order 3d finite elements?

I am familiar with finite element simulations and numerical schemes like the Gauss Legendre quadrature. I also know that the Gauss integration yields exact values for polynomials up to degree 2n-1...

27 February 2021 6,818 3 View

High aspect ratio hexahedral mesh optimization solver?

I am interested in evaluating mesh optimization solvers that converge to high aspect ratio unstructured hexahedral grids. In other words, I am interested in a solver that optimizes warpage,...

26 February 2021 7,279 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View