What is the reason, we got both test and control band on LFA in negative sample?

I am working on Lateral flow assay for virus detection by carbon conjugated polyclonal antibody. We got a high intense band in both test and control line on LFA in negative sample used as buffer....

11 February 2021 6,558 2 View

How Learning in Multiagent Control System Using Graph Theory is useful ?

I would like to know application of learning and control in MAS setting. And where could I find simulation codes of learning in MAS control

04 January 2021 1,866 4 View

How to fix the problem about segmentation fault?

Hi everyone, I am new gromacs user. I am doing the MD simulation of protein-NA. I did the Energy minimization step, NVT, NPT equilibration. after equilibrating the system i submitted the...

21 August 2020 7,876 4 View

How to extract MODIS AOD data at a location from multiple hdf files?

I have MODIS hdf files for number of days. I want to extract the AOD data at a time for particular location.

09 July 2020 1,544 3 View

Anyone can help me for How to solve the dangling problem in DNA?

Hi, I working on Molecular Dynamics simulation of DNA. I want to study the 5'phosphate attached to thymine 16 number residue in my pdb file. I added these 5' Phosphate attached thymine in...

08 March 2020 8,195 1 View

Could anyone please provide a protocol for conjugation of antibodies to gold nanoparticles?

Could anyone please provide a protocol for conjugation of antibodies to 30 nm gold nanoparticles?

20 November 2019 9,369 4 View

How to determine if a white colored precipitate on the surface of an extruded aluminium sample (multi-port tube) is aluminium hydroxide?

Dear researchers, I have some multiport extruded tubes with a certain white precipitate visible on the iner side of the tubes and would like to know a way to determine what that precipiate...

10 November 2019 3,318 7 View

How we can keep steel tube specimen freeze at -40 degree Celsius at room temperature for a hours?

without chamber for fatigue testing

24 January 2019 5,676 3 View

Which solvent is suitable to dissolve pressmud sample...?

I am working on pressmud and its combinations for making good bio fertilizer for agriculture use.............

03 January 2019 2,756 4 View

Suggest guidelines for selecting learning rate of neural network?

Suggest thumb rules.

05 February 2018 3,578 4 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View