I have been trying to determine the correct equation for calculating the energy expenditure from the readouts of the CLAMS caging data. I have VO2 (mL/kg/hr), VCO2 (mL/kg/hr), and RER. I have found a paper (attached) that uses the equation 15,818(VO2) + 5,176(VO2)/1000*24. The CLAMS equation sheet (attached) calls for the equation (3.815 + 1.232 * RER) *VO2. There are two prongs to my question - how should I properly calculate energy expenditure from this data, and how are the constants for these other equations derived?
Hi Jessica
I am using the CLAMS equation, and the value for energy expenditure is automatically calculated in my result sheet (called "Heat"). Then I normalize this value using the amount of lean mass for each mice. In the end you get a value in cal/hour/kg of lean mass
I suggest you stop normalizing by team mass along and start using ANCOVA.
I agree with Nelson. With our group, we recently commented this on Nature Methods
Fernández-Verdejo, R., Ravussin, E., Speakman, J.R., Galgani, J.E., 2019. Progress and challenges in analyzing rodent energy expenditure. Nat Methods. https://doi.org/10.1038/s41592-019-0513-9
- Badges
- Science topic