Importance of DO2 in Columbus Instruments CLAMS cages?

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How to calculate energy expenditure from CLAMS caging data?

I have been trying to determine the correct equation for calculating the energy expenditure from the readouts of the CLAMS caging data. I have VO2 (mL/kg/hr), VCO2 (mL/kg/hr), and RER. I have...

08 September 2017 6,236 3 View

Can I leave out the permeabilization step in immunofluorescence microscopy?

Our protein of interest is membrane-bound, and we have been toying with the idea that we may be able to tweak our protocol in order to cut out the permeabilization step and therefore get more...

01 February 2016 1,398 2 View

Who is the best custom antibody supplier?

My lab is currently looking at creating a custom antibody. There aren't a lot of customer reviews on the websites for these things, so what are the companies that you all prefer to synthesize your...

06 July 2014 2,210 6 View

How do I know if a protein is knocked out?

A classmate of mine tested nicastrin knockout and wildtype cells against antibodies, but never tested to make sure that the knockout cells did not indeed contain nicastrin. So far, I have...

05 June 2014 7,457 10 View

Is there a way to preserve HRP-conjugated seconday antibodies in blocking buffer without using sodium azide?

I recently read that using sodium azide to preserve the HRP-conjugated secondary antibodies in their blocking buffer will inactive the HRP-conjugation. Is there another preservative that I can add...

05 June 2014 5,785 6 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View