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Questions related from Jessica Hatter
I have been trying to determine the correct equation for calculating the energy expenditure from the readouts of the CLAMS caging data. I have VO2 (mL/kg/hr), VCO2 (mL/kg/hr), and RER. I have...
09 September 2017 6,371 3 View
I am new to analyzing the CLAMS data from the metabolic caging from Columbus Instruments. We have been looking @ VCO2, DCO2, VO2, DO2, RER, and activity as markers of metabolic aberrations....
08 August 2017 5,323 2 View
Our protein of interest is membrane-bound, and we have been toying with the idea that we may be able to tweak our protocol in order to cut out the permeabilization step and therefore get more...
02 February 2016 1,499 2 View
My lab is currently looking at creating a custom antibody. There aren't a lot of customer reviews on the websites for these things, so what are the companies that you all prefer to synthesize your...
07 July 2014 2,263 6 View
A classmate of mine tested nicastrin knockout and wildtype cells against antibodies, but never tested to make sure that the knockout cells did not indeed contain nicastrin. So far, I have...
06 June 2014 7,530 10 View
I recently read that using sodium azide to preserve the HRP-conjugated secondary antibodies in their blocking buffer will inactive the HRP-conjugation. Is there another preservative that I can add...
06 June 2014 5,870 6 View
