In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

What is the suitable range of Sulfur content and Viscosity of Heavy fluid Oil that used in Kiln?

What is the disadvantages of high sulfur content to the ceramic burning process? and what is the effect of outranged viscosity?

24 February 2021 8,219 2 View

Solution of 2D convection diffusion heat equation using MATLAB ?

This is a polymer heating problem. Polymer enters at around 180C (x=0) in the barrel. Also barrel initial temperature is 180 (t=0). Some heat Is added along whole length of barrel q. We have to...

15 February 2021 3,887 1 View

Defining effective diffusivity in porous zone and non-porous zone?

Hi all, I am working on methanol steam reforming using packed bed for hydrogen generation. I am using FLUENT software for CFD simulation. In reference paper, authors mentioned that effective mass...

10 February 2021 6,104 1 View

How to match the XRD of a compound for which there is no JCPDS file ? Is there any other characterization to claim the synthesis of desired compound?

I have synthesized LiMgF3 using the starting precursors LiF and MgF2 via solid state diffusion method. Now I am looking for some characterization to claim the desired product, as there is no...

09 February 2021 921 3 View

Is it possible to have a diffusion of oxygen from the glass substrate (SiO2) into the absorbent layer during annealing process?

02 February 2021 382 7 View

How do you determine at what concentration of antimicrobial should you subject the pathogen to?

I am making an antimicrobial extract from fungal metabolite but the fungi are not known. So I don't know at what concentration I should subject them to but what I do know I will test them against...

07 January 2021 897 6 View

Has anyone done lab models on the influence of dead-end pores on mass transport in saturated flow?

Groundwater flows through voids and passages that are interconnected within porous media. Some of the pores are referred to as a dead-end pore space within the porous matrix. The presence of...

07 January 2021 3,590 3 View

Any recommendation to calculate time to dominant design emergence?

Any recommendation to calculate time to dominant design emergence?

01 January 2021 3,809 5 View

Are drones following the e-Auto industry technological trajectory or are they following its own?

* Are drones following the e-Auto industry technological trajectory or it is following its own? * Will drones diffuse faster than electrical vehicles? * Will it depend on the application?, What...

29 December 2020 7,379 3 View

How can I apply the eddy_parameter to the other dataset?

I have one DTI dataset, and I've applied two different reconstruction algorithms to it. Thus there were two datasets with the same anatomical structure but varying in sth like the artifacts and...

28 December 2020 2,745 3 View

How best can Agricultural Innovation be structured to ensure adequate uptake and farmers adoption?

Technology adoption rates amongst small-scale farmers in the Global South (e.g. Africa) remain low, even when scientific evidence shows that a particular technology is beneficial.

28 December 2020 4,786 23 View