I used qiagen maxi prep kit for plasmid isolation, but I have a issue in the pellet. Though it is a high copy number plasmid, very less pellet is obtained. Is there a reason for it?
How to calculate CFU/m3 in settle plate?
14 July 2024 1,816 1 View
I would like to explores the connection between behavioural economics, emotional models, and consumer psychology to design an applied neuromarketing practice. The study aims to bridge the gap...
11 June 2023 1,536 9 View
Smartbox or energy is an important item of a solar power system. I would be grateful if someone can help me/suggest a literature source to find life cycle inventory of the smart box. Thanks.
25 November 2020 1,839 1 View
Polyurethane insulating foam plays key role in energy-efficient cooling systems such as storage in cold stores, local storage in supermarkets, and domestic refrigerators. In LCA assessment of...
13 November 2020 6,514 3 View
In recent years a new development happened in solar refrigerator design by eliminating expensive and problematic energy storage batteries. The technology so-called, direct-drive refrigeration...
06 November 2020 3,366 3 View
Is it possible to get a small heater 30*30 mm size, which can work up to 200 degrees C? As part of my research, I need a small heater 30*30 mm size with the thermostat, which can work up to 200...
15 September 2020 8,987 3 View
I am working on circular polarization, can you help me how to plot axial ratio with respect to frequency and radiation patterns for a resonance frequency?
24 April 2020 7,745 0 View
I have YMnO3 (yttrium manganese oxide) in powder or pellet form.
09 December 2019 2,632 0 View
I am working on designing of microstrip lines and its behavior with ebg structures. i am unable to find the design equations for microstip line length and width for a particular resonance frequency.
23 September 2019 5,773 1 View
I am looking for any user friendly online tools for predicting the secondary structure for 3'UTR.
23 August 2019 2,379 7 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,081 3 View
I am not able to get good literature and the physics behind how first these grains and grain boundaries arises out of no where when we make a pellet to study its dielectric properties and then how...
07 August 2024 5,176 3 View
I am trying to prepare samples for IP. The pellet size is good and I am using 1% Tx-100 and using a syringe to further lyse the cells and maintain protein-protein interactions. I keep having a...
01 August 2024 7,199 1 View
I'm fairly new to making homemade comp cells and have done so with some mild success. What is generally an acceptable way of resuspending comp cells during wash steps? I've been told to swirl the...
31 July 2024 8,309 0 View
I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...
22 July 2024 5,953 6 View
How to determine tannin content in fodder pellets?
22 July 2024 8,161 3 View
I am extracting proteins from plant samples and am currently attempting to use the TCA/Acetone extraction method. I am having trouble resolubilizing my sample after drying the pellet. I collected...
15 July 2024 9,025 3 View
Someone advise me to use 1 to 2mm undersized pellet but it is not available. Do anyone have any experience in the field of tunnel kiln ?
28 June 2024 6,355 0 View
Hi, I´d like to trace the C and N flow in a recirculating aquaculture system by labelling the fish feed with concentrated stable isotopes. From a supplier, I found a product ouf of algae lysate...
19 June 2024 746 0 View
I've pelleted down the overnight ampicillin supplemented cultures of DH5ALPHA for pET22b isolation 5 days ago and stored them on -20°C without adding any buffer or glycerol. Would the cells be...
09 June 2024 6,478 2 View