How to extract water soluble amino compound from after aqueous work up?

4-Dimethyl amino pyridine is used in reaction.Product formed contains amino group along with amide & is highly water soluble. To remove 4-DMAP aqueous work up done. Please give solution. Thanks.

21 February 2021 1,883 1 View

Can any one suggest me good journals for "plant disease detection using machine learning" ?

any SCI,SCIE, and Scopus index ( or simillar) journals of relevant field

17 December 2020 7,148 4 View

How to perform Rietveld refinement of doped bulk powder sample using GSAS-2?

I have XRD data of a doped powder sample. How can I perform the Rietveld refinement using GSAS-2. When I use cif file of doped powder, symmetry is lost in the refining process. How to perform...

10 December 2020 9,717 4 View

How can we turn a short peptide having affinity for some compound into whole cell biosensor?

Greetings, I have a short stretch of 20 aa with strong binding affinity for a compound. I am trying to de some whole cell flourescence or visible biosensing technique based on this peptide. Can...

26 November 2020 8,322 3 View

Can i check superlattice period for tio2 thin film made via spin coating ?

Can i check superlattice period for tio2 thin film made via spin coating ? superlattice period is seen only in MBE or can be there in tio2 coated on glass via spin coating ??

20 September 2020 3,748 3 View

Can graphite rods moderator in nuclear waste as a radio isotope thermo-electric generator or beta decay energy harvester as mini battery or capacitor?

Can we use the graphite moderator rods from nuclear waste in the mini reactor ,beta voltage cell decay ? or we can use the thermo-electric generator is there any possibility? for some miniature...

15 September 2020 1,726 8 View

I have got the following photo luminescence spectra data of AU-PD tio2 film for diffrent depotion rates ,can some one help me to analyse it broadly?

i have got the following photo luminescence spectra data of AU-PD tio2 film for diffrent depotion rates ,can some one help me to analyse it broadly? can we discuss it ??

02 September 2020 3,260 6 View

Does SrTiO3 substrate makes any diffrence when we do coating of TiO2 or any other material ?

I have deposited on glass surface SrTiO3 . I want to deposit TiO2 over it via spin coating for further studies . Will I observe any difference in properties ? or Can I use this substrate for...

24 August 2020 6,654 3 View

What input parameters are need to be provided while Validation of results of Experimental Tension test in ANSYS Workbench?

I have created new material in ANSYS Workbench with parameters obtained from the experimental tension test. I have provided the data of Stress strain, E, Syt, Sut, density, poissions ratio while...

13 August 2020 5,458 5 View

Should one put position restraint on protein backbone during simulations?

Hi, I am studying a transporter protein. X ray crystallographic structure is not known yet. So, i am using homolgy modelled structures for my studies. I am curious if i should put position...

30 July 2020 3,191 1 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

What is the proper in vitro culturing method to get information about the activation of innate immune cells by VLPs?

Dear All, I am interested in studying the response of immune cells against virus-like particles (VLPs). I used the PBMC in vitro assay and treat it with the VLP using PHA as a positive ctrl while...

16 February 2021 1,337 2 View

Why does flow deformation occur at the test line and control line on nitrocellulose membranes (LFIA)?

I use 1% BSA in 10 mM phosphate buffer (pH 7.4) solution when striping my nitrocellulose membranes with antigens and antibodies. However, I always observe deformed flow when my sample or running...

16 February 2021 4,224 3 View

Can the viability assays (ex: MTT/CCK-8) measure cell proliferation? or are they solely viability assays?

I want to conduct a proliferation assay to test a chemotherapy combination on a cancer cell line. At first, I thought about the MTT assay. But now, all sources are saying that these do not measure...

14 February 2021 3,142 7 View

GST assay in microplate?

I'd like to know if anyone can provide me with a protocol for traditional GST enzymatic assay (with GSH and CDNB as substrates) in a 96 well microplate, for cell line and/or C.elegans...

11 February 2021 1,328 3 View

What lysis buffer is compatible with Bradford assay?

Hello! I am planning to use Bradford assay to measure protein content in PBMCs lysates. The problem is that Bradford assay doesn't seem to be compatible with Tris-based buffers or SDS. Are there...

10 February 2021 1,797 5 View