How to get Proteinase K resistant bands of PrPSc from ScN2a lysate by western blot?

I have already tried different lysis buffers and many protocols also. But, still I am not getting consistent result.

07 February 2020 650 3 View

Can someone suggest what type of microscope would be best for imaging human hematopoietic colonies on MethoCult?

I am currently inducing human pluripotent stem cells towards the hematopoietic lineage and assessing the functional capacity of the progenitors by performing colony based assay on MethoCult. I see...

19 February 2019 1,562 3 View

Will the availability of high speed internet be a major drawback in IoT development and implementation?

This is also a part of my work... So i want more about this in details. Please help me by giving answer of this question in details......... THANK YOU......

10 January 2019 8,102 3 View

Can anyone help me how to solved two nonlinear coupled differential equations using matlab?

I have attached the equations

05 January 2019 7,955 5 View

Has anyone used Lipofectamine LTX + Plus Reagent for transfection in HEK 293Tcells?

Tell me about your experiences in transfection please, thanks!

09 December 2018 8,886 2 View

When transmittance is less than 10% how to measure absorbance accurately?

We have developed a coated fabric which can be used as UV protector. We are in a process to optimize the parameters of the fabric for the highest UV light absorbency. Surprisingly, we are getting...

01 November 2017 8,936 3 View

Why can't we replace x-rays with electron beam?

14 July 2016 2,241 4 View

ZnO 1-D nanostructures gives best UV result with which solvent?

ZnO 1-D nanostructures gives best UV result with which solvent?

11 December 2015 1,170 3 View

After immunisation in chickens of a recombinant antibody, I am not getting positive antibody titres. What is wrong?

12 September 2015 5,295 7 View

Is there any effect of the spraying process on nanostructured YSZ thermal barrier coating?

spraying processes such as APS ,HVOF Which one is the best for TBC and why ?

15 April 2015 2,406 1 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How can I make stacked bar graph with scatter plot?

I want to make a stacked bar graph with showing each data value as dots. I tried with graph pad prism, version 6, but could not succeed. Does anyone have tried before making such graphs?

01 March 2021 9,383 1 View

What could be reason for getting such type of graph for complex impedance in case of polymer blend films?

Its effect appears in real part of ac conductivity also. Can anyone suggest any reason behind it?

26 February 2021 3,487 3 View

How to use measure capacitance change in COMSOL time dependent study ?

I want to measure change of capacitance due to relative displacement between two parallel plates in COMSOL. Distance between two plates changes with time. So I'm using a time dependent study but...

26 February 2021 8,567 3 View

Can I use a graph from a another source in a thesis?

I have a permission from X company to use their graph from a report, but they have a reference in the bottom left corner of the graph as follows: "Source: Database Y". Is it OK to use this graph...

24 February 2021 2,269 3 View

I have need of XRD graph analysis software for phase identification. Can you please provide links for software tools which can be downloaded?

i am trying to download x'pert high score software, but can't download it. please do suggest software for phase analysis

24 February 2021 5,687 4 View

Could you please suggest to me research in which a relation between the shear wave velocity and the degree of saturation for stone was discussed?

Hi all, I am looking for a graph or a relation that relates the degree of saturation and the shear wave velocity for the marlstone. Looking forward to your suggestions Best Regards

24 February 2021 9,690 1 View

How to find the capacitive response frequency from the impedance plot?

I have done electrochemical impedance by using cyclic voltammetry for one organic material. from that, I plotted bode diagram. but actually, I am very confused about how to find the capacitive...

18 February 2021 3,023 3 View

Algorithms to filter corrective max and min from a set of data?

I've got this 5 year Direct Normal Irradiance data I downloaded from https://re.jrc.ec.europa.eu/. I ordered this by year and by every 24 hours because I need the DNI curve by max in summer, min...

17 February 2021 2,777 5 View

There is any numerical criterion for identifying neighbours for Voronoi /Gabriel graphs?

If there is a set of n points in a plan (with known x, y coordinates), can I use a numerical criterion to establish if two points are neighbours, given the definition of a neighbour for Voronoi...

17 February 2021 6,914 4 View