Dear all,

I need your help for buffer calculation. Authors gave the buffer components as final concentrations as below. I'd like to prepare 10ml of that buffer. So, how much volume of each component I need to add to my buffer?

Thanks.

Buffer 1: 15 mM Tris-HCl (pH 7.4–7.6), 25 mM NaCl, 5 mM MgCl2,15 mM Na2HPO4, 2.5 mM EDTA, 1%sucrose

Buffer 2: pH=8.8; 6%SDS, 3 mM MgCl2,15 mM Tris-HCl, 0.5%DMSO, 6%acetone