We have been trying to find Ka from EQNMR software but were unable to because of its non-compatability with Windows10 , so if you can suggest some other software or method to find Ka.
For this, the best option available is the SpecFit program (http://goo.gl/y1ekqL) that it's kind of expensive. There is a free alternative (for academia), which is called DynaFit (http://goo.gl/yDzFnJ). DynaFit is powerful yet simple to use and very flexible, you can put there any binding mechanism you want (1:1, 1:2, mixed 1:2+1:2, etc.) and it will fit your data. The instructions aren't that user-friendly, but with the aid of the examples (they are included in the download package) I'm sure you can learn how to use it. So, I'd suggest you give a try to DynaFit.
Specfit: http://www.kingkongsci.co.uk/specfitglobalanalysis.htm
See attachments for papers.
https://www.researchgate.net/post/Which_software_can_be_used_to_determine_dissociation_constant_for_enzyme-ligand_binding_using_QUADRATIC_equation_as_shown_in_the_pic_attached
https://www.researchgate.net/post/How_do_I_calculate_a_dissociation_constant_using_MATLAB2
Other software:
http://app.supramolecular.org/bindfit/
http://community.dur.ac.uk/j.m.sanderson/science/downloads.html
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