How to remove the salt and get purified DNA in Silica Magnetic bead-based Nucleic acid extraction?

I am doing nucleic acid extraction (manual silica magnetic bead-based) from the serum sample. After the extraction, I have taken the OD 260/280 in UV spec. There is no protein contamination. But...

04 November 2020 1,061 2 View

Could you tell me the composition of the wash buffer in RNA extraction (Silica Magnetic Bead-based) and its proportions?

I am doing silica magnetic beads-based RNA isolation. I am facing some impurity issues because of washing. So kindly share some best washing buffer composition of wash buffer for especially for...

01 November 2020 6,536 2 View

Best book for Fluid Structure Interaction?

Practical methods along with the basic behind Fluid Structure Interaction.

11 June 2020 9,743 9 View

Most of the Research papers are found more theoretical and previous work but only few paragraph spent in experiment and result , Why ?

My research is going on Traffic analysis , Prediction and Intelligent Transport System. I have good concept in theory but I found that most of the papers are not elaborated experiments setup...

17 April 2020 407 12 View

Can I use Phosphate Buffer for Antibody and Fluorescence conjugation?

I am going to use Phosphate Buffer (pH 6.5) is an alternative of MES buffer (pH 6.5) for Antibody and Fluorescent Microspheres conjugation procedure. The literature I am referring to mentions MES...

22 December 2019 794 3 View

IoT Challenges with Healthcare?

In want do some work in the security of IoT with Healthcare , please suggest me some area , in which area our scholar can do some work. If possible provide some detail from 2018 and 2019 Dr....

12 July 2019 6,628 3 View

Performance Analysis of Big Data ?

How performance of the Big data can be analysis, which framework should used ? Is Hadoop and Spark Framework are good or other tool should use , is any Source for code or algorithm can find?

01 July 2019 348 3 View

I want to study about antibody antigen binding for chikunguniya and malaria virus? Where can i find relevant material?

Study for Virus diagonesis

23 April 2019 9,210 5 View

Address of Tanusri Mandal is wrong . How to rectify?

Tanusrei Mandal, an author of the paper, entitled "Calcite solubilization by Bacteria: An Novel method of Environmental pollution control ". Published in Geomicrobiology Journal Vol.32, PP.846...

23 January 2018 1,566 3 View

Bandgap Engineering of BTO/bottom electrode/STO (or any suitable substrate) for Photovoltaic Applications.

I am interested in reading some literatures on the Bandgap Engineering of BTO/bottom electrode/GSO (or any substrate), could someone provide me some links of interesting papers on the same?

06 August 2017 3,989 2 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

How do I determine the degree of polymerization of PEG 400 based on NMR integration?

Hello, I am working on the photocatalytic degradation of PEG 400. I would like to know if it's possible to determine the degree on polymerization based on NMR integration, or by the MW of the...

02 March 2021 6,186 3 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

Can polymer concentrations be calculated based on the number average molecular weight?

Hello, I spin-cast two different polymers onto wafers and measure their height as a function of initial concentration. For polymer A, I have the weight-average molecular weight (Mw) as well as...

28 February 2021 7,882 2 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

How to reduce residual stresses in MD simulation?

I am working on a preparation of representative polymeric structures of some popular polymers, such as polyimide or PTFE for MD simulation, during which a single nitrogen or oxygen atom will be...

24 February 2021 8,105 3 View

How to identify what type of fillers are in a polymer?

Hello everyone, I have some PHAs blended with fillers and nucleating agents, yet I don't know what kind of fillers or nucleating agents. The producer has shut down and there's no way to get in...

24 February 2021 1,356 8 View

How can I selectively dry etch Ti on a polymer substrate?

24 February 2021 5,378 1 View

How to do a surface treatment of uncoated TiO2 with polymer?

Basically, I would like to know how to treat or coat on the uncoated bare TiO2 (Ti-OH rich) surface with polymer which has anchoring groups in the polymer structure like trimethoxygroups. What is...

21 February 2021 4,657 6 View