How to increase DPI in my image?

I'm submitting manuscript to a journal that requires 1200 dpi in my images. I have changed the dpi from 96 but when I upload the image with 1200 dpi, the image looks super blurry when I review my...

16 February 2021 2,803 3 View

Did I dilute my supernatant too much so cytokines will be below the limit of detection on a Luminex assay?

I am performing an experiment analyzing the cytokine production of macrophages in response to viral infection. I recently harvested and cultured peritoneal macrophages from mice. I plated these...

17 January 2021 5,516 3 View

What are some examples of practice based methods and techniques that can be used to study social dance styles?

I am currently developing a project where the primary mode of inquiry is rooted in practice based methods and techniques. The project is focused on a social dance form. I have read several...

23 March 2020 793 5 View

Does POM1 cause precipitation with ATP?

Hi, I've been doing multiple experiments to measure POM1 inhibition of ATP breakdown by CD39. I measure the amount of breakdown using either the malachite green assay or the ATP luciferase...

17 February 2020 5,340 1 View

How does being a teacher influence creative processes in creating choreography?

I would be interested in resources that pertain to how identity or the different roles we possess in dance making (dancer, teacher, spectator, mentor, etc.) influence creative processes in...

06 December 2019 8,064 2 View

Can anyone recommend any articles with clear ubiquitin staining in cells?

My ubiquitin staining is fairly variable and I've been looking for published articles containing such stainings so I can see how it normally looks. I've had some trouble locating any good sources...

14 September 2019 8,904 1 View

Western blots on tissue which have been stored in RNAlater?

I have used up my snap frozen tissue from an experiment but have some left in RNAlater (invitrogen). I was wondering if anyone had any experience with running western blots using tissue that has...

28 August 2019 7,930 3 View

Ecological Validity: How can experiments that take place in a social setting measure validity and reliability?

Thinking in terms of a social setting such as a dance, a concert, a meal, if an experiment were to be designed in such a way, how can the method be validated? Similarly, what role would...

26 August 2019 3,415 3 View

Does the volume of a song influence our enjoyment of it?

If a song is played louder does it increase or decrease our enjoyment towards a song? Do we have to already enjoy or dislike a song in order to notice the influence of volume? Any possible...

22 August 2019 2,089 11 View

What are the best conditions for transferring a >200kDa and ~40kDa protein?

I am having issues transferring my large protein (220kDa) at 30V for 2 hours (RT). It is often patchy with incomplete bands (annoyingly I occasionally I get a clear transfer!). For my other...

22 August 2019 1,081 7 View

Heat exchanger with liquid Nitrogen as coolant?

I want to cool Natural Gas from 195 Kelvin to 138 Klevin in a heat exchanger by using liquid nitrogen as the coolant medium. When entering the heat exchanger, the liquid nitrogen will have a...

01 March 2021 6,450 5 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

How the wettability of rock sample can be changed by ageing in pure hydrocarbon oils only?

I want to do wettability analysis of hydrocarbon oil (octane)/rock/surfactant solution system. but to do the analysis first I have to make rock surface oil-wet. Since hydrocarbon oil does not have...

27 February 2021 5,383 3 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

What is the suitable range of Sulfur content and Viscosity of Heavy fluid Oil that used in Kiln?

What is the disadvantages of high sulfur content to the ceramic burning process? and what is the effect of outranged viscosity?

24 February 2021 8,219 2 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View