I'm trying to amplify Salmonella phage to a titer of greater than log 9 pfu/ml. Currently my collection sits at around log 6 pfu/ml...is there any way to do this quickly and efficiently?
you can try PEG precipitation to concentrate your phage sample. look at
http://www.abdesignlabs.com/technical-resources/bacteriophage-preparation/
As mention above you can concentrate by centrifugation after PEG precipitation in a sucrose or CSCl gradient or use amicon columns for concentration of your initial stocks. This method https://peerj.com/articles/2261/ describes a "phage on tap" method for production of high titre stocks. Much of the method described can be adjusted to solve your problem
Dear Karen,
From my long years of phage experience: liquid cultures of lytic phages do not produce large amounts of virions !? However, if you grow them on plates using the double layer technique, you can scratch the upper layer with a microscope support glass into a 50 ml tube, add a small volume of buffer or else and vortex it very well to get the phage particles out of the agar mess. The supernatant will contain a very large number of phage particles (the smaller the amount of buffer added the larger concentration of phages to get!!!). Try it, it works ! So you don't have to mess with PEG, sucrose and ultracentrifuges....Good luck
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