How to avoid getting red serum after centrifugation of blood samples collected from retroorbital plexus of rats ?

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Joachim hans Misterek

dear Sara,

its a haemolysis or red cell contamination?

by red cell contamination you can start a second zentrifugation step to deplete the RBC

by haemolysis store the blood after collection by 2 hours by room temperatue follow a overnight storage by 4-8° C before prozessing

rgds

Gregory Barshtein

Probably, at the stage of blood sampling you provoke RBC hemolysis. Try using a needle with larger diameter.