How to avoid chimera formation in PCR?

More Kamesh Krishnamoorthy Kalyankumar's questions See All

Between Julia and MATLAB, which would be the most efficient for a discontinuous Galerkin FEM code?

I need to simulate a 2-D problem with DGFEM. I have been advised to use C/C++ for a code that delivers quick and efficient results, however, I am not very comfortable with them, and my base code...

16 December 2019 7,001 5 View

Is the Zero Shot Learning a good current research area or not?

I am going to do my research on Zero Shot Learning. Is it good area in recent trends...

03 December 2018 7,101 3 View

Increased manganese availability in flooded soils?

plants like paddy dont suffer from manganese deficiency since they are grown in flooded soils? what is the reason for the increased availability of manganese in flooded soils?

14 April 2018 6,421 2 View

Any simple protocol for whitefly DNA extraction?

Iam finding it hard to get amplification post whitefly DNA extraction using lysis buffer method. Any other simple methodology. What are the precautions or troubleshooting measures to be followed?...

09 March 2018 3,103 4 View

Insects for DNA extraction stored in ethanol post collection?

Generally insects like whiteflies collected for DNA extraction are stored in ethanol. Does ethanol act as a preservant. Does it help in maintaining the integrity of the insect DNA. How long till...

17 February 2018 452 2 View

Viral like RNA assemblies?

Sequencing of plant transcriptomes often gives rise to additional sequences called virus like RNA assemblies corresponding to genomic RNAs of viruses infecting these host plants. How the plants...

05 February 2018 9,009 1 View

What does the word canonical mean with respect to translation?

I came across a term like "non canonical translation in RNA viruses". What does the term canonical mean?...even though after a dictionary search I havent got a satisfied answer. Does the word...

27 January 2018 6,184 4 View

Sodium acetate storage?

Should 3M sodium acetetate prepared for molecular cloning be stored under refrigerated conditions? what is the reason if so?

06 January 2018 6,301 2 View

Dilution of antiserum and conjugate in ELISA?

During an ELISA test for plant virus, if the quantity of antiserum and conjugate is less, is it possible to dilute the antiserum and conjugate with water (ultrapure/milliq)?

03 January 2018 8,665 3 View

Dominant and codominant marker with reference to PCR primer?

Is there any term such as dominant and codominant marker in relation to a PCR primer

29 December 2017 8,780 3 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View