How to avoid chimera formation in PCR?

More Kamesh Krishnamoorthy Kalyankumar's questions See All

What does an "Error 111" mean on an AKTA Explorer?

Dear Colleagues, I would be grateful if any of you can advise me on what an "error 111" is on an AKTA Explorer. The manual suggests that the instrument be turned off and turned on. That does not...

13 June 2024 2,961 1 View

Where do I obtain Chenopodium quinoa Willd. (quinoa) seeds (SAMN04338310; QQ74) that were originally used for genome sequencing?

The Chenopodium quinoa Willd. (quinoa) seeds with accession PI614886 (Biosample accession code SAMN04338310; also known as NSL 106399 and QQ74) is what I require and was also used for genome...

10 December 2023 5,680 2 View

What dilutions of serum sample should I use in ELISA?

I would like to do an ELISA to measure the levels of a biomarker in mouse serum. The concentration of the standards ranges from 2000pgs/ml to 31.25pgs/ml. I have the kit from R&D systems but...

18 February 2022 10,013 2 View

Sequence identity matrix?

While generating a sequence identity matrix in bioedit software by pasting the sequences from notepad, often there is an overlapping of sequence names (given before the fasta sequence) and the...

10 January 2020 1,339 0 View

Between Julia and MATLAB, which would be the most efficient for a discontinuous Galerkin FEM code?

I need to simulate a 2-D problem with DGFEM. I have been advised to use C/C++ for a code that delivers quick and efficient results, however, I am not very comfortable with them, and my base code...

16 December 2019 7,151 5 View

Is the Zero Shot Learning a good current research area or not?

I am going to do my research on Zero Shot Learning. Is it good area in recent trends...

03 December 2018 7,235 3 View

Increased manganese availability in flooded soils?

plants like paddy dont suffer from manganese deficiency since they are grown in flooded soils? what is the reason for the increased availability of manganese in flooded soils?

14 April 2018 6,544 2 View

First aid measure for Ethidium bromide spill?

If there is an accidental ethidium bromide spill on skin/hands during a moelcular work what is the immediate first aid measure to be taken using very simple chemicals available in the lab?

12 March 2018 443 3 View

Any simple protocol for whitefly DNA extraction?

Iam finding it hard to get amplification post whitefly DNA extraction using lysis buffer method. Any other simple methodology. What are the precautions or troubleshooting measures to be followed?...

09 March 2018 3,232 4 View

Insects for DNA extraction stored in ethanol post collection?

Generally insects like whiteflies collected for DNA extraction are stored in ethanol. Does ethanol act as a preservant. Does it help in maintaining the integrity of the insect DNA. How long till...

17 February 2018 667 2 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View