hi guys, I am doing experiments with cornea epithelial cell line. In my experiment, I have to wash away the residual drug in cells with PBS, and I use 96 wells plate(flat bottom). After the washing procedure, I always find a lot of cells are washing away along with the drug. The intensity of the cell is about 80%-90% in the well before I do the experiment. And the number of losing is hard to control so it affected the experiment results. I want to ask if there are any tips or suggestions for the washing procedure on 96 well plates? Thank you, guys.
I am wondering whether you have experienced such cell loss only on the 96-well plate or also on other flasks or dishes. Anyway, given that your cell line can attach and grow well on the plate but in the absence of the drug, pre-coating the bottom of wells, and spinning down the plate before aspiration may help. If detachment from the bottom is due to drug toxicity, its treatment protocol may need to be modified, e.g., at a longer period of time at a lower concentration.
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