I am doing autoclaving for sterilization of glassware. After the sterilization, everything seems to be fine. However I let it cool in the fume hood and somehow moisture accumulates in my glassware. I need them to be dry afterwards. Any suggestions?
My suggestions would to be to sterilize tapped glassware with cotton,. Another option could be putting smaller parts into a closed and large container use your procedure without opening the container.
glassware keep it in plastic bag n seal the bag . Autoclave after autoclaving take it Laminar airflow open plastic cover Keep in UV for 20min use plates :)
There are several options. It seems that if you autoclave on a dry cycle, those problems should be resolved unless you are in a high humidity environment. Autoclave in paper sterilizing bags, allow the normal cool down period and store in a cool dry environment. There should be no condensation using this method and if there is, have the autoclave checked.
Alternative is to heat sterilize glass ware. This can be cone by covering any opening with aluminum foil and placing it in an drying oven at 160° C for 2 - 2.5 hours. If you are sterilizing Pasteur pipettes with cotton plugs, careful not to keep at that temperature for longer than 2 hours as the cotton will burn if left too long. Dry heat indicators are useful to identify glassware that has been heat sterilized as their indicator strips change color once exposed to the sterilizing temps.
Carmelo suggestion is right if you use a dry cycle it should be dry. However I keep the glassware in drying cabinet post sterilization.
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