Which statistical test can I use to test whether two standardized regression coefficients differ significantly?

Hello, I have conducted a multiple regression with two predictor variables and would like to find out whether the difference between the standardized regression coefficients is significant....

22 June 2020 8,788 11 View

Class imbalanced dataset for Machine Learning - how to test it?

I have a data-set with good and malicious files. Labelled as 0 and 1. In the data-set there are mor malicious files - like 3000 vs 800 good files. Nevertheless I am getting good results with...

27 April 2020 3,752 14 View

How to design a dataset of files and their "issues" for machine learning classification?

I have a few thousand files and each file has multiple "issues" after being deconstructed. They are labelled 0 or 1 for good or malicious. I think these "issues" can be a feature to determine if...

02 April 2020 2,941 2 View

Factorial Survey Experiment Analysis?

2x2x2 Design

26 November 2019 4,808 3 View

Has anyone experiences with drying plant parts (leaves, stems, roots, ears, kernels), especially of wheat plants in domestic microwave ovens?

Dear all, we need a procedure to rapid drying plant materials from field or greenhouse experiments to rapidly denaturing enzymes, kills micro-organism and stabilizes the tissues. On one hand we...

31 October 2018 5,942 9 View

No title

At the moment we hear a lot about artificial intelligence and machine learning. It is an awesome development, unimaginable completely or partially, now. How and when humans will gain the benefits...

19 September 2018 801 25 View

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21 July 2018 2,931 3 View

For NGS, can I use samples prepared with differerent number of cycles of PCR?

I am preparing samples for NGS, RNA sequencing. I isolate total RNA from B. subtilis, deplete it from rRNA and then make DNA libraries. Every step is quality-checked using BioAnalyser. And in the...

08 April 2018 7,871 6 View

Detaching cells from Lab-Teks. Do you have any suggestions?

Does anybody have any experience with cells detaching from Lab-Teks and how to deal with it? In one of our experiments we are not able to use gelatin or anything else. In other experiment we are...

13 September 2017 4,777 1 View

Bax protein isolation

19 June 2017 5,329 3 View

No title

02 March 2021 3,060 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View