How to analyze and statistically compare the two proliferation curves derived from IncuCyte when the curves are not starting at the same initial cell density (confluence) point? How to normalize them?
Hello What are the experiences and perspectives of nurses providing nursing care at home and in the community for patients in palliative care?
12 June 2024 2,236 5 View
A joint presentation I am involved in is published on researchgate without my name. What is the policy here?
29 October 2023 256 1 View
Hello, I would like to ask you for help. I am trying to find genotyping protocol for roy a9 and slc45a2 b4 zebrafish mutants. Does anyone know them? Thanks.
26 October 2023 4,046 0 View
Dear administrator, before I started working for the Uniklinikum in Erlangen, I was employed in Hamburg, Uniklinikum. After the long maternal leave I forgot I had an account already. I would like...
14 August 2023 1,483 2 View
Hello all, appreciate your help as it is much needed. I am trying to understand whether my thesis should be a research paper or a case study. I want to study the effects of disruptive events of...
09 July 2023 1,933 6 View
Dear all, I would appreciate a lot if anyone could give me an advice how to resolve problem with non-speciffic labelling of immunofluorescently-lablelled mouse brain free floating sections, which...
03 December 2022 9,604 2 View
Dear colleagues, from a practical point of view, how do I accurately measure sediment pH of more or less compacted, anoxic lake sediments in the field? Imagine there is a freshly recovered and...
28 July 2022 2,012 8 View
If any of you have worked with the MM28 cell line (uveal melanoma metastasis) how did you freeze it to maintain the best cell viability?
08 May 2022 4,400 1 View
Comprehensive ground water management requires suitable tools that consider the groundwater balance as well as the variety of potential risks that might impact groundwater recharge and chemistry....
30 December 2021 6,849 3 View
I am currently working doing a lot of N/N 2D gel electrophoresis for investigating replication structures in yeast. We might invest in a bead beater, which can handle a specific volume, but I am...
21 September 2021 2,596 4 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Are there any statistical methods to justify your sampling technique using SPSS or AMOS?
05 August 2024 9,153 4 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View