To determine microsatellite instability in a cancer patient sample, it is necessary to compare the parallel samples i.e. tumor tissue and peripheral blood. How the microsatellite instability or stability is determined in a cell line?
Is it possible to analyse MSI in cell lines using monomorphic MSI markers, where individual to individual variations in sequences are absent?. In that case, can the MSI markers in peripheral blood from healthy individuals serve as controls?.
Hi, Microsatellite instability can be detected by mononucleotide markers by a simple PCR, or detection of mismatch repair proteins (MLH1, PMS2, MSH2 and MSH6) by IHC.