Hi everybody!
I need to amplify phage DNA so as to be able to produce restriction profiles using different restriction endonucleases.
Can anyone recommend which primers should I use? I know random primers are of choice, but to choose amongst commercially available primers is proving quite hard! Any suggestions? Brand and reference would be greatly appreciated!!!
It is probably easier to infect ecoli cells and grow cultures which will generate any amount of lambda dna
https://groups.molbiosci.northwestern.edu/morimoto/research/Protocols/I.%20Prokaryotes/B.%20Phage%20Preparation/1.Bacteriophage%20Prep.pdf
Victor M. Balcão what is it that you are attempting to do? I don't think random primers are going to let you determine the restriction map of the phage genome, but it isn't clear if that is your goal. As Paul Rutland mentioned, it might be easiest to just amplify your phage (not PCR) and prepare a good stock of phage DNA. Or it might be easiest to just sequence the genome of the phage and have all the information you might need from that.
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