Victor M. Balcão

3 Questions 2 Answers 0 Followers

Questions related from Victor M. Balcão

How to differentiate between plaques of lysis from phages from those of endolysins?

We are in the process of determining the title and making one-step growth curves for lytic phages, but we are at a point where dilutions up to 10ˆ-54 result in complete lysis.... no counting of...

18 April 2023 717 4 View

How can I distinguish experimentally a lytic from a lysogenic phage?

I must experimentally demonstrate beyond any doubt that isolated phages are lytic and not lysogenic... Of course, if the phage DNA is sequenced, one can look for the presence of integrase...

11 September 2020 8,537 3 View

How to amplify phage DNA (dsDNA) for producing restriction profiles?

Hi everybody! I need to amplify phage DNA so as to be able to produce restriction profiles using different restriction endonucleases. Can anyone recommend which primers should I use? I know random...

07 August 2020 1,373 3 View