Formulate model for one integer optimization?

I am working on one optimization problem. the model with two states, the transition between the two states based on one threshold value (L = 5, which L< 5 -> Normal state, L== 5 ->...

11 February 2021 4,045 3 View

The impact of different poisson's ratios when analysising field vibration?

The Poisson's ratio of soil is quite difficult for us geo-engineers to obtain. I came across a problem when I tried to determine the parameters in a FEM model. For the saturated clay in...

02 January 2021 8,971 3 View

What does a higher LoD indicate in context of COVID19?

I have seen COVID19 test LoD ranging from 180 to 600000 NAAT Detectable Units/mL So a rapid antigen test with LoD of TCID50 1.13 x10^2 per mL and RT PCR TCID50 1x10^-2 per mL (600 NDU/mL) and a...

28 November 2020 1,238 3 View

How to use CEL technology in ABaqus to simulate TNT explosion in air?

Hello everybody! Recently, I have been using CEL technology to simulate the TNT explosion in the air, but I have encountered some difficulties. I don't know how to do discrete field material...

13 September 2020 7,012 2 View

Why cannot the flow cytometry be applied to the detection of rare events?

"Flow cytometry was a very rapid and accurate method but it could not be applied to the detection of rare events" I read this in the following article, but there is no explanation...

18 July 2020 6,400 3 View

KCL, NaCl crystalline, NH4Cl, PEG in MOPS with BSA and Procline 300?

Why do we need all three salts in MOPS buffer? and PEG added to the reagent. NaCl crystalline 1.359 g/L KCl 0.708 g/L Ammonium chloride 0.0063 g/L KCL...

26 April 2020 6,080 4 View

Do you think current guidance for COVID-19 prevention is correct?

CDC provided guidance to the public "Wash hand, disinfect surfaces, cover sneezing" ever since the outbreak of COVID-19. I get a strong sense this guidance is for contact transmitted disease...

01 April 2020 8,279 1 View

Primer Specificity SARS-COV, 2019nCOV?

I have attached the screen shot. In the table below It compares sequences between COVID-19, SARS-COV and Bat COV Mismatch numbers 11 and 9, meaning that COVID-19 compared to the other two...

30 March 2020 9,084 3 View

Competitive immunoassay prinicple?

A competitive immunoassay technique is used. This involves the reaction of anti-HBc in the sample with hepatitis B core antigen (HBcAg) coated wells. Unbound material is removed by washing....

16 March 2020 9,894 3 View

SAC Well Coating?

The wells are polystyrene coated with streptavidin via a biotin BSA linking layer using the SAC well coating method. What is SAC coating method?

06 March 2020 3,016 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View