Is there a simple (that is one that requires few and not very exotic reagents) chemical reaction to "activate" or "functionalize" the polystyrene of a simple microtitre plate?
In other words, I would like to add carboxyl groups to the surface of a common ELISA plate to then use ethyl-carbodiimide hydrochloride and N-hydroxysuccinimide to immobilize a target on the bottom of the well.
Luigi Marongiu
Please look at the following link ; will provide you the details of immobilization of proteins on the polystyrene base of the plate :
https://www.corning.com/catalog/cls/documents/application-notes/CLS-DD-AN-454.pdf
Hello, the note only talks in general about activation, it essentially says to buy Cornig's plates. There is no actual chemical reaction to make them in house...
Look at the normal ELISA procedure and work on those buffers accordingly !!
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