Can the same primer pair , say for instance ITS 1 and ITS 4 generate different molecular weight amplicon after PCR for different fungal species?

Say Fungus A gives 500 bp and Fungus B gives 550 bp is it an absurd result?

29 December 2020 881 3 View

If a certain paper cited is written language other than English, does its interpretation in another authors work become the baseline for it?

A paper not written in English , does it need to be read in its original form before being cited by anyone in the paper that they are writing . Or it is enough to view its interpretation in...

11 October 2020 4,280 8 View

Is it okay to use PCA regression on a time series dataset?

I have a dataset of a binary response variable and 22 predictor variables that have multicollinearity. My goal is to make a predictive model. The data was sampled every 30 seconds over three days....

08 October 2019 5,746 2 View

How to do time series analysis with one/few missing data points?

I have three datasets given to me of images of a conveyor belt taken every 30 seconds over 3 days, but one image was removed/lost in one of them and a few (~10-20) in the others. I calculated the...

02 October 2019 3,893 5 View

Do you think fruit sugar is harmful for human health?

We hear regularly from health organization and experts that we should eat less sugar. As we know that fruit is also valuable source of sugar. So, consumption of more fruit is harmful for human...

22 September 2019 6,270 6 View

How to culture cancer cells the right way?

I am relatively new to cell culturing and I am currently working with several lines of cancer cells, some of them being HeLa, A549, HCT116, etc. I have some very basic doubts regarding the...

03 September 2019 3,003 3 View

How to characterize surface roughness using stereomicroscope pictures when the two samples are different colors (one is much darker)?

I am using the stereomicroscope to generate stacks to capture each plane of the leaf in focus. I am using ImageJ and the plugins SurfCharJ 1q and Fraclac to quantify the leaf roughness and fractal...

04 July 2019 2,282 3 View

Why are XRD images black and white?

XRD images or diffraction spots are black and white. SEM images too. In fact, I think any atomic scale image is black and white. Why are they colorless?

10 April 2019 4,563 3 View

How to extract total protein from strawberry fruit?

I have extracted protein from strawberry by 4% SDS+65mM DTT buffer and found good band in SDS PAGE but when I performed western blot, i didn't observed protein band. What is the reasons? I have...

03 April 2019 7,547 3 View

How to estimate the marginal effects of Bivariate Probit Models in STATA?

Dear All, I am running a Bivariate Probit model in STATA and tried to estimate the marginal effects; however, every time its showing some error message. Could you please help me to run the exact...

25 March 2019 4,637 6 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

How to find the mode of classified ocean colour images?

Hello, I have classified 10 S3 OLCI images from the same area (althouth not the same size and/or quadrant) and I would like to do a final image using the mode of the pixel of these 10 images. I...

01 March 2021 9,874 3 View

How to threshold an image despite bright lighting, using ImageJ/Fiji?

Hi everyone, I am using Fiji to determine the percentage of the image covered by leaves, branches, or trunk, but i've encountered some difficulty when the sun hits parts of my image like the one...

27 February 2021 1,194 3 View

Trouble with Charting with Google Earth Engine (Technical)?

Hello. I have a problem with the charting in google earth engine. I created an image collection by combining 5 image collections of five parameters and using ee.Join.inner ( ) and .apply ( )...

26 February 2021 7,084 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View