Breast Cancer Immunohistochemistry (IHC) slides analysis?

I want to know how to look/analyze different parts in a tumor section like stroma, Ductal epithelial, epithelial lining, luminal epithelial etc etc in a breast cancer tissue slide. Also is there...

02 June 2020 5,050 3 View

Semi Dry vs Wet Transfer recipe?

Hi I found that there are two different recipes for transfer depending on the mode (Semi-dry or wet) of the transfer. Why is it so? If anybody has prior experience with the use of both the...

26 December 2019 946 3 View

Double delta Ct values given by PCR machine according to Applied biosystems Real Time system?

I performed real-time PCR using an Applied biosystems machine. The double delta Ct values ( according to the machine) for the gene of interest after certain treatments were given as -5.12454;...

23 November 2019 5,286 3 View

Molecular weight of protein is low but signal is at higher molecualr weight ?

The mol wt of my protein of interest is about 27 Kda but i am getting the signal at approx 50Kda. I am Using polyclonal antibody and the standard 12% gel for the detection. Running gel conditions...

02 June 2019 8,599 5 View

Cells adhering at the edges of the culture flask/dishes?

Hi I have noticed one thing in cell culture is that the cells love to attach themselves at the side of the the culture vessels more than at any other part of the flask. Also, the these cells...

27 August 2018 1,708 4 View

HBL-100 Normal or Normal like or transformed?

Hi I am confused regarding the status of HBL 100. Some mention it as 'normal' cell line while others state that it is 'normal like' and another is it is 'transformed'. A clear and precise...

23 August 2018 3,805 3 View

RIPA lysis buffer?

Hi everyone I made a fresh RIPA buffer using foll. composition 1) Tris Buffer 50mM (7.5 pH) 2)150mM NaCl 3)1%NP-40 4)0.5% Sod. deoxycholate 5)Protease inhibitor After lysate was run on SDS-PAGE...

10 June 2018 9,067 5 View

Can bacteriophages be used against biofilm forming within indwelling medical devices in-vivo?

14 August 2017 4,098 3 View

How to give 2 separate material orientations after partitioning a body, in Abaqus CEL analysis?

I am currently trying to model a coupled temperature-displacement analysis of a CFRP using Abaqus 6.14-3. As the mesh distortion is very high, I am using the CEL (Coupled Euler Lagrangian)...

08 August 2016 2,079 3 View

Which kit is good for isolation of circulating tumor DNA (ctDNA) from blood?

Which kit has shown good results so far in order to get good quantity of circulating tumor DNA.? what average amount of circulating DNA can we expect from cancer patients blood (plasma and serum)?

13 March 2016 6,338 3 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

Is stone a sustainable material? Is it a sustainable approach to use natural stone waste in architecture?

I'm designing a zero-waste restaurant and considering using marble waste to make some tables and a bar countertop. My clients think it's not "sustainable enough" and I would like to have more...

01 March 2021 4,326 7 View

How Can I bring as much common elements in steel as possible to calculate stable phase(s) in OpenCalphad?

As I was tweaking step-by-step OpenCalphad6 Macros step1.ocm involving phase and thermal property calculation of a High-speed steel, I could not incorporate more than 6 elements in the steel...

01 March 2021 8,840 1 View

How the wettability of rock sample can be changed by ageing in pure hydrocarbon oils only?

I want to do wettability analysis of hydrocarbon oil (octane)/rock/surfactant solution system. but to do the analysis first I have to make rock surface oil-wet. Since hydrocarbon oil does not have...

27 February 2021 5,383 3 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

What is the error of the Txy diagram?

Hi, I know the attached Txy diagram has an error but can't figure it out. Could you please look at the diagrams and explain it to me?

23 February 2021 4,239 1 View

How to get/count refractive index in gasses?

20 February 2021 8,202 6 View

What is the best procedure for measuring the pH of aqueous colloidal systems?

There has long been a debate among my colleagues about measuring the pH of aqueous colloids. As I see there is no general truth in the matter, so I ask this question below. What is the best and...

31 January 2021 8,203 3 View

How to prevent aggregation of nano-particles without using surfactants?

As it is well known fact that nano-particles can easily aggregate during nucleation/ synthesis part due to high surface energy..by reading literature, I came to know that surfactants can help to...

30 January 2021 8,206 6 View

How do you annotate figures to show significance?

30 January 2021 1,008 3 View