I want to purify PCR product.
I used XBaI and KPnI enzymes.
Now I want to make reaction mixture.
From the universal buffer table it is showing that I have to use 1xM
What should I understand from this information? How to calculate?
Usually suppliers will provide the buffer with higher concentration, for example 5X or 10 X. You need the final concentration on 1X in your reaction.
so if its 50 ul reaction and your buffer is 10 X use 5.0 ul to get 1X final concentration.
if its 5X then use 10 ul.
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