How the result of copy number variants be interpreted using real-time PCR techniques?

12 December 2012 1 9K Report

I am very new to the concept of copy number variants. Say for eample, If I wanted to look copy number variants in Salivary alpha amylase (AMY1 gene) using real-time PCR technique, how can we determine If there is a deletion or duplication?

Badges
Science topic

More Shanthi Duraimani's questions See All

RNA extraction from Buccal swab?

I am going to isolate RNA from Buccal swab. I have experience in extracting RNA from frozen blood and PBMC. But, I wanted to know which RNA extraction kit will be appropriate? Can I extract...

03 April 2013 5,910 9 View

How to write author name in the methodology for research article?

I am going to publish a paper in an International journal, where they follow APA style. I have some query in writing author name in the methodology. I have used one of the scientists protocol...

31 December 2012 2,328 4 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View