How to measure rod/spindle shaped particle size distribution from FESEM images?

FESEM image showing dense sindle like geometry. Need to measure the particle size distribution.

22 June 2020 3,136 1 View

What is typical range of Fermi energy in Graphene? and Relation between conductivity and Fermi energy of Grpahene?

Conductivity has an inverse relation with Fermi velocity (VF). But Fermi velocity (VF) is directly proportional to Fermi energy.

18 June 2020 8,140 2 View

How to get Proteinase K resistant bands of PrPSc from ScN2a lysate by western blot?

I have already tried different lysis buffers and many protocols also. But, still I am not getting consistent result.

07 February 2020 650 3 View

Which is the website for eBURST analysis of MLST data?

I have done MLST analysis for my isolates on the pubmlst website. I am trying to do the eBURST analysis for the allelic profiles I got from the MLST analysis. I checked a number of papers for the...

06 February 2020 6,464 3 View

How to deal with missing X,Y,Z co-ordinates of particular atom in ligand .cif file ?

I am trying to prepare parameter files for G7M modified residue for MD simulation using Amber. I got G7M.cif from RCSB PDB but in that file co-ordinates for H0P3 are missing which is causing an...

07 October 2019 7,801 2 View

NF Kb estimation: any available biomolecule in serum which can estimate the expression of NF-KB?

nuclear factor kappa light chain enhancer of activated B cells which gives estimate of cytokine release, ultimately inflammation . Is there any diagnostic kit is available which can estimate its...

28 July 2019 1,864 5 View

How to confirm the RNA sequence for any mutation after in vitro transcription?

I have to do in vitro transcription of ssDNA then PCR and using PCR product for transcription by T7 polymerase of repeat containing RNA.So after purification and denaturing gel electrophoresis ,...

31 May 2019 3,161 2 View

Has anyone used Lipofectamine LTX + Plus Reagent for transfection in HEK 293Tcells?

Tell me about your experiences in transfection please, thanks!

09 December 2018 8,886 2 View

Which parameter does control stiffness degradation in ABAQUS Concrete Damaged Plasticity Model for triaxial Loading case?

I am trying to verify the experimental results on triaxial compressive loading on concrete cylinder using Concrete Damaged Plasticity Model, but I am not able to get results as expected.. Getting...

16 September 2018 2,765 5 View

Is pAc5.1 plasmid low copy number plasmid?

I consistently get low yields with pAc5.1 constructs. I have tried growing cultures from fresh plates and glycerol stocks. I have also tried to use starter cultures (grown for 8 h) and I always...

05 June 2018 8,204 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View